Isolation of UVR8 Protein Complexes

Isolation of UVR8 Protein Complexes

The basic mechanism of gentle regulated plant growth includes photoreceptors and their interacting proteins which act as gentle signaling intermediate components. In Arabidopsis thaliana, UV RESISTANCE LOCUS 8 (UVR8) is liable for the notion and the initiation of UV-B gentle sign. To knowledge, only some proteins have been revealed because the elements of UVR8 protein complexes, limiting our understanding of the molecular mechanisms by which UV-B gentle enter is interpreted to orchestrate quite a few physiological outputs in crops. Therefore, it’s essential to isolate and determine the elements of UVR8 protein complexes at a worldwide stage, with the intention to uncover novel UV-B gentle signaling components and pathways. In this chapter, we offer a protocol for the isolation of UVR8 protein complexes. Basically, co-immunoprecipitation (co-IP) assay is employed to counterpoint UVR8 and its associating proteins in vivo. This technique can be utilized coupling with particular remedies and is appropriate with successive biochemical evaluation.

Recently the vaginal route take into account as a super route for drug supply methods (DDS) administration. This is as a result of, it’s appropriate for decrease drug dosage, increased drug focus within the genital tract tissues and decrease drug focus in pregnant ladies blood circulation. However, the vaginal route administration faces many challenges because of the physiology in addition to the complexity of vaginal tissue histology. Here on this examine, throughout diestrus stage (optimum situation for overseas substance internalization), single or twin measurement of fluorescent thiol-organosilica nanoparticles (tOS-NPs) had been administrated intravaginally. The biodistribution and reactivity of tOS-NPs in several tissues of the feminine genital tract had been investigated beneath the fluorescence microscope.

Furthermore, utilizing immunohistochemical staining, the expression of F4/80 protein and the function of macrophages in transport and re-location of tOS-NPs from vaginal lumen into completely different genital tissues or different organs had been investigated. This examine confirmed that, tOS-NPs measurement and kind of tissue are vital in biodistribution and uptake of tOS-NPs within the genital tract. Small measurement (100 nm) of tOS-NPs was extremely collected within the genital tract tissues particularly endometrial epithelium in contrast with giant tOS-NPs (1000 nm). Contradictory, the massive measurement induced the expression of F4/80 protein and the quantity of vaginal macrophages in contrast with small measurement. However, each small and huge sizes of tOS-NPs had been discovered co-localized with F4/80+ macrophages, positioned within the vaginal, endometrial and ovarian tissues. The tOS-NPs intravaginally administrated had been discovered within the splenic tissues, indicating its potential to enter the blood circulation from the vaginal lumen.

Additionally, the excessive accumulation of tOS-NPs within the endometrial epithelium indicated the endometrial first go impact of tOS-NPs. As a consequence, excessive focus of tOS-NPs within the endometrial epithelium might scale back the focus of tOS-NPs-based DDS within the blood circulation and their unwanted effects. Furthermore, throughout vaginal tissue optimum situation (diestrus stage), understanding the destiny and biodistribution of tOS-NPs will introduce vital knowledge concerning the growth of save and efficient DDS for the pregnant ladies

Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry

Accurate abundance measurements of mobile proteins are required to attain a quantitative and predictive understanding of any organic course of contained in the cell. Existing strategies to find out absolute protein abundances are labor-intensive and/or require refined experimental and computational infrastructure (e.g., fluorescence correlation spectroscopy (FCS)-calibrated imaging and quantitative mass spectrometry). Here we element an easy stream cytometry-based technique to measure absolutely the abundance of any Halo-tagged protein in dwell cells that makes use of a typical mammalian cell line with a identified quantity of Halo-CTCF proteins just lately characterised in our lab.
The protocol solely includes just a few steps. First, a cell line expressing the Halo-tagged protein of curiosity is grown and labeled side-by-side with our normal line. Then, common fluorescence intensities are measured by typical stream cytometry evaluation and at last a easy calculation is utilized to estimate absolutely the quantity of the Halo-tagged protein of curiosity per cell. Once the protein of curiosity has been endogenously tagged with HaloTag, which we routinely obtain by Cas9-mediated genome modifying, the offered protocol is quick, handy, reproducible, cost-effective and readily accessible.
Isolation of UVR8 Protein Complexes

ERα down-regulates carbohydrate responsive factor binding protein and reduces cardio glycolysis in liver most cancers cells

Deregulated metabolism is one of the traits of hepatocellular carcinoma. Sex hormone receptor signalling has been concerned within the marked gender dimorphism of hepatocellular carcinoma pathogenesis. Oestrogen receptor (ER) has been reported to cut back the incidence of liver most cancers. However, it stays unclear how oestrogen and ER regulate metabolic alterations in liver tumour cells. Our earlier work revealed that ERα interacted with carbohydrate responsive factor binding protein (ChREBP), which is a transcription issue selling cardio glycolysis and proliferation of hepatoma cells. Here, the information confirmed that ERα overexpression with E2 therapy decreased cardio glycolysis and cell proliferation of hepatoma cells. In addition to modestly down-regulating ChREBP transcription, ERα promoted ChREBP degradation.
ERα co-immunoprecipitated with each ChREBP-α and ChREBP-β, the 2 identified subtypes of ChREBP. Although E2 promoted ERα to translocate to the nucleus, it didn’t change subcellular localization of ChREBP. In addition to interacting with ChREBP-β and selling its degradation, ERα decreased ChREBP-α-induced ChREBP-β transcription. Taken collectively, we confirmed an unique function of ERα in suppressing cardio glycolysis in liver most cancers cells and elucidated the mechanism by which ERα and ChREBP-α collectively regulated ChREBP-β expression.

Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells

Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells

Protein evaluation in bone marrow samples from sufferers with a number of myeloma (MM) has been restricted by the low focus of proteins obtained after CD138+ cell choice. A novel method based mostly on capillary nano-immunoassay may make it doable to quantify dozens of proteins from every CD138+ purified MM pattern in an automatic method. Up to now, the information of protein degree in these cells was restricted as a result of a comparatively small amount of pattern is on the market after the diagnostic process. Moreover, the pattern usually is required for nucleic acids evaluation.

We have developed the process for acquiring proteins from bone marrow samples preserved in RLT+ buffer, and now we have efficiently utilized this method for the quantification of proteins within the setting of sufferers with MM. Proteins are extracted from RLT+ buffer, the content material is quantified by whole protein assay with WES machine and eventually, the actual protein expression degree is evaluated utilizing particular antibodies by capillary nano-immunoassay with WES machine. The current protocol permits us to quantify many proteins from a restricted quantity of pattern, with out shedding the chance to acquire nucleic acids on the identical time. Proteins are quantified robotically in an assay with a low chance of human errors, which makes it a useful gizmo for biomarkers improvement.

Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization

Numerous experimental approaches exist to check interactions between two subunits of a big macromolecular complicated. However, most strategies don’t present spatial and temporal details about binding, that are crucial for dissecting the mechanism of meeting of nanosized complexes in vivo. While latest advances in super-resolution microscopy methods have supplied insights into organic buildings past the diffraction restrict, most require in depth experience and/or particular pattern preparation, and it’s a problem to increase past binary, two colour experiments.

Using HyVolution, a super-resolution method that mixes confocal microscopy at sub-airy unit pinhole sizes with computational deconvolution, we achieved 140 nm decision in each dwell and stuck samples with three colours, together with two fluorescent proteins (mTurquoise2 and GFP) with important spectral overlap that had been distinguished by means of shifting the excitation wavelength away from widespread wavelengths. By combining HyVolution super-resolution fluorescence microscopy with bimolecular fluorescence complementation (SRM-BiFC), we describe a brand new assay succesful of visualizing protein-protein interactions in vivo at sub-diffraction decision. This methodology was used to enhance our understanding of the ordered meeting of the Saccharomyces cerevisiae spindle pole physique (SPB), a ~1 giga-Dalton heteromeric protein complicated fashioned from 18 structural parts current in a number of copies. We suggest that SRM-BiFC is a robust device for examination of direct interactions between protein complicated subunits at sub-diffraction decision in dwell cells.

Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells

Interaction between Borrelia miyamotoi variable main proteins Vlp15/16 and Vlp18 with plasminogen and complement

Borrelia miyamotoi, a relapsing fever spirochete transmitted by Ixodid ticks causes B. miyamotoi illness (BMD). To evade the human host´s immune response, relapsing fever borreliae, together with B. miyamotoi, produce distinct variable main proteins. Here, we investigated Vsp1, Vlp15/16, and Vlp18 all of that are at present being evaluated as antigens for the serodiagnosis of BMD. Comparative analyses recognized Vlp15/16 however not Vsp1 and Vlp18 as a plasminogen-interacting protein of B. miyamotoi. Furthermore, Vlp15/16 certain plasminogen in a dose-dependent vogue with excessive affinity.
Binding of plasminogen to Vlp15/16 was considerably inhibited by the lysine analog tranexamic acid suggesting that the protein-protein interplay is mediated by lysine residues. By distinction, ionic energy didn’t impact binding of plasminogen to Vlp15/16. Of relevance, plasminogen certain to the borrelial protein cleaved the chromogenic substrate S-2251 upon conversion by urokinase-type plasminogen activator (uPa), demonstrating it retained its physiological exercise. Interestingly, additional analyses revealed a complement inhibitory exercise of Vlp15/16 and Vlp18 on the choice pathway by a Factor H-independent mechanism. More importantly, each borrelial proteins shield serum delicate Borrelia garinii cells from complement-mediated lysis suggesting a number of roles of these two variable main proteins in immune evasion of B. miyamotoi.

Mouse Anti Feline Leukaemia Virus P27 Monoclonal Antibody

DMABT-51281MF 0.25 mg
EUR 767

Goat Anti Feline Leukaemia Virus P27 Polyclonal Antibody

DPBT-66847GF 1 ml
EUR 861

Feline Leukemia Virus p27 antibody

10-1520 100 ug
EUR 337
Description: Mouse monoclonal Feline Leukemia Virus p27 antibody

Feline Leukemia virus p27 antibody

10-F05A 250 ug
EUR 308
Description: Mouse monoclonal Feline Leukemia virus antibody

Feline Leukemia Virus p27 antibody

10-F05C 250 ug
EUR 217
Description: Mouse monoclonal Feline Leukemia Virus p27 antibody

Feline Leukemia Virus p27 antibody

20-FG32 1 mg
EUR 128
Description: Goat polyclonal Feline Leukemia Virus p27 antibody

Feline Leukaemia Virus gp70 (FeLV gp70) Antibody

abx413483-025mg 0.25 mg
EUR 592
  • Shipped within 1 week.

Feline Leukaemia Virus p15E (FeLV p15E) Antibody

abx414340-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

Feline Leukemia Virus p27 protein

30-1928 100 ug
EUR 457
Description: Recombinant FeLV p27 protein with his tag

Feline Leukemia Virus p27 antibody (HRP)

60-5006 1 ml
EUR 409
Description: Goat polyclonal Feline Leukemia Virus p27 antibody (HRP)

Feline Leukemia Virus p27 antibody (biotin)

60-5007 1 ml
EUR 378
Description: Goat polyclonal Feline Leukemia Virus p27 antibody (biotin)

Mouse Anti Feline Leukaemia Virus P15e Monoclonal Antibody

DMABT-51278MF 0.25 mg
EUR 767

Mouse antibody for Feline Leukemia virus FeLv p27

7218 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Mouse antibody for Feline Leukemia virus FeLv p27

7226 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Mouse antibody for Feline Leukemia virus FeLv p27

7228 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Feline Leukemia virus antibody

10C-CR1297M1 100 ug
EUR 247
Description: Mouse monoclonal Feline Leukemia virus antibody

Feline Leukemia Virus gp70 antibody

10-F07A 250 ug
EUR 327
Description: Mouse monoclonal Feline Leukemia Virus gp70 antibody

Feline Leukemia Virus gp70 antibody

10-F07B 250 ug
EUR 327
Description: Mouse monoclonal Feline Leukemia Virus gp70 antibody

Feline Panleukopenia Virus PCR kit

PCR-V171-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline Panleukopenia Virus

Feline Panleukopenia Virus PCR kit

PCR-V171-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline Panleukopenia Virus

Feline immunodeficiency virus PCR kit

PCR-V175-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Feline immunodeficiency virus

Feline immunodeficiency virus PCR kit

PCR-V175-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline immunodeficiency virus

Feline leukemia virus PCR kit

PCR-V176-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Feline leukemia virus

Feline leukemia virus PCR kit

PCR-V176-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline leukemia virus

Goat polyclonal antibody for Feline Leukemia virus

274 1 ml
EUR 321.88
Description: This is HRP conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus

7221 1 ml
EUR 283.81
Description: This is goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus

7223 1 ml
EUR 300.13
Description: This is FITC conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus

7227 1 ml
EUR 300.13
Description: This is Biotin conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Feline Herpes Virus Type 1 (FHV1) Antibody

abx414268-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

Cat Leukemia Virus p27 Protein

20-abx260224
  • EUR 885.00
  • EUR 342.00
  • EUR 1609.00
  • 0.5 mg
  • 100 ug
  • 1 mg
  • Shipped within 5-10 working days.

Recombinant Feline Immunodeficiency Virus Core Antigen

DAG552 100ug
EUR 949

Native Feline Infectious Peritonitis Virus antigen

DAGA-3038 1mg
EUR 1040

Feline Immunodef. virus core recombinant antigen

8963 100 ug
EUR 561.13
Description: This is Feline Immunodef. virus core recombinant antigen for ELISA,WB.

Feline Panleukopenia Virus RT PCR kit

RTq-V171-100D 100T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline Panleukopenia Virus .

Feline Panleukopenia Virus RT PCR kit

RTq-V171-150D 150T
EUR 701
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline Panleukopenia Virus .

Feline Panleukopenia Virus RT PCR kit

RTq-V171-50D 50T
EUR 532.8
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline Panleukopenia Virus .

Feline immunodeficiency virus RT PCR kit

RTq-V175-100R 100T
EUR 776.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline immunodeficiency virus .

Feline immunodeficiency virus RT PCR kit

RTq-V175-150R 150T
EUR 877.9
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline immunodeficiency virus .

Feline immunodeficiency virus RT PCR kit

RTq-V175-50R 50T
EUR 631.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline immunodeficiency virus .

Feline leukemia virus RT PCR kit

RTq-V176-100R 100T
EUR 776.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline leukemia virus .

Feline leukemia virus RT PCR kit

RTq-V176-150R 150T
EUR 877.9
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline leukemia virus .

Feline leukemia virus RT PCR kit

RTq-V176-50R 50T
EUR 631.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline leukemia virus .

Mouse antibody for Feline Leukemia virus FeLv gp70

7217 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv gp70 for WB, ELISA.

Mouse Anti-Feline Leukemia Virus (FeLV) monoclonal antibody

CABT-BL8814 1.0 mg
EUR 892

Mouse Anti-Feline Leukemia Virus (FeLV) monoclonal antibody

CABT-BL8815 1.0 mg
EUR 892

Feline Immunodeficiency Virus p24 Antigen (FIV p24) Antibody

abx411336-02mg 0.2 mg
EUR 592
  • Shipped within 1 week.

Feline Immunodeficiency Virus gp41 Antigen (FIV gp41) Antibody

abx411337-02mg 0.2 mg
EUR 592
  • Shipped within 1 week.

Feline Immunodeficiency Virus p24 Antigen (FIV p24) Antibody

abx412988-2ml 2 ml
EUR 606
  • Shipped within 1 week.

Feline Immunodeficiency Virus (F1a), RNA Aptamer, unlabeled

AR-230-U Custom Ask for price

Feline Panleukopenia Virus One-Step PCR kit

Oneq-V171-100D 100T
EUR 747.4
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline Panleukopenia Virus.

Feline Panleukopenia Virus One-Step PCR kit

Oneq-V171-150D 150T
EUR 838.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline Panleukopenia Virus.

Feline Panleukopenia Virus One-Step PCR kit

Oneq-V171-50D 50T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline Panleukopenia Virus.

Feline immunodeficiency virus One-Step PCR kit

Oneq-V175-100R 100T
EUR 933
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline immunodeficiency virus.

Feline immunodeficiency virus One-Step PCR kit

Oneq-V175-150R 150T
EUR 1060.6
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline immunodeficiency virus.

Feline immunodeficiency virus One-Step PCR kit

Oneq-V175-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline immunodeficiency virus.

Feline leukemia virus One-Step PCR kit

Oneq-V176-100R 100T
EUR 933
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline leukemia virus.

Feline leukemia virus One-Step PCR kit

Oneq-V176-150R 150T
EUR 1060.6
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline leukemia virus.

Feline leukemia virus One-Step PCR kit

Oneq-V176-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline leukemia virus.

Feline Immunodeficiency Virus gp95 Envelope Antigen (FIV gp95) Antibody

abx411335-02mg 0.2 mg
EUR 592
  • Shipped within 1 week.

Feline Immunodeficiency Virus gp95 Envelope Antigen (FIV gp95) Antibody

abx414342-025mg 0.25 mg
EUR 592
  • Shipped within 1 week.

P27 antibody

70R-16333 50 ul
EUR 435
Description: Rabbit polyclonal P27 antibody

p27 antibody

70-PR78 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal p27 antibody

p27 antibody

70R-13747 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal p27 antibody

p27 antibody

20-PR78 100 µL
EUR 755
Description: Rabbit polyclonal p27 antibody

p27 Antibody

DF6090 200ul
EUR 304
Description: p27 Antibody detects endogenous levels of total p27.

p27 Antibody

abx149167-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

p27 Antibody

ABD6090 100 ug
EUR 438

Feline FeLV antibody

10-2328 100 ug
EUR 325
Description: Mouse monoclonal Feline FeLV antibody for lateral flow

Feline FeLV antibody

10-2329 100 ug
EUR 325
Description: Mouse monoclonal Feline FeLV antibody for lateral flow

Feline Immunodeficiency Virus p24 gag Antigen (FIV p24 gag) Antibody

abx413943-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody

20-abx006544
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVC2) Antibody

abx034469-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVC2) Antibody

abx034469-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody

20-abx322439
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody

20-abx322440
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Feline Calicivirus (FCV) Antibody

abx413481-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

p27 Polyclonal Antibody

41299-100ul 100ul
EUR 252

p27 Polyclonal Antibody

41299-50ul 50ul
EUR 187

p27 Polyclonal Antibody

41300-100ul 100ul
EUR 252

p27 Polyclonal Antibody

41300-50ul 50ul
EUR 187

p27 Kip1 antibody

70R-49527 100 ul
EUR 287
Description: Purified Polyclonal p27 Kip1 antibody

p27 Kip1 antibody

70R-49528 100 ul
EUR 244
Description: Purified Polyclonal p27 Kip1 antibody

p27 Kip1 Antibody

20-abx009454
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

p27 Kip1 Antibody

20-abx123451
  • EUR 592.00
  • EUR 857.00
  • EUR 411.00
  • 100 ul
  • 200 ul
  • 50 ul