Protein-protein interactions represent the molecular foundations of nearly all organic processes. Co-immunoprecipitation (CoIP) experiments are most likely essentially the most broadly used technique to probe each heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that a number of nuclear proteins akin to transcription components are spatially distributed into native high-concentration clusters in mammalian cells, suggesting that many nuclear proteins self-interact. These observations have additional underscored the necessity for orthogonal biochemical approaches for testing if self-association happens, and if that’s the case, what the mechanisms are. Here, we describe a CoIP protocol particularly optimized to check self-association of endogenously tagged nuclear proteins (self-CoIP), and to judge the position of nucleic acids in such self-interaction. This protocol has confirmed dependable and sturdy in our fingers, and it may be used to check each homotypic and heterotypic (CoIP) protein-protein interactions.
The ASR protein household has been found thirty years in the past in lots of plant species and is concerned within the tolerance of numerous abiotic stresses akin to dehydration, salinity and warmth. Despite its significance, nothing is thought concerning the conserved ABA-Water Deficit Stress Domain (ABA-WDS) of the ASR gene household. In this examine, we characterised two ABA-WDS domains, remoted from durum wheat (TtABA-WDS) and barley (HvABA-WDS). Bioinformatics evaluation exhibits that they’re each persistently predicted to be intrinsically disordered. Hydrodynamic and round dichroism evaluation point out that each domains are largely disordered however belong to totally different structural courses, with HvABA-WDS and TtABA-WDS adopting a PreMolten Globule-like (PMG-like) and a Random Coil-like (RC-like) conformation, respectively. In the presence of the secondary construction stabilizer trifluoroethanol (TFE) or of rising glycerol concentrations, which mimics dehydration, the 2 domains purchase an α-helical construction. Interestingly, each domains are capable of stop heat- and dehydration-induced inactivation of the enzyme lactate dehydrogenase (LDH).
Follistatin secretion is enhanced by protein, however not glucose or fats ingestion in overweight individuals independently of earlier gastric bypass surgical procedure
The DEAD-box protein household of RNA helicases: sentinels for a myriad of mobile capabilities with rising roles in tumorigenesis
Polystyrene-bottomed 96-well plate (ELISA) |
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CT361 | U-CyTech | 6 | EUR 87.6 |
24 Well Solid Bottom Assay Plate - PK100 |
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PCR0352 | Scientific Laboratory Supplies | PK100 | EUR 511.65 |
96-Well Filter Plate |
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FCM-B01M | ACROBIOSYSTEMS | 1plate | EUR 21.4 |
Description: The membrane of this plate has a consistent physical structure with a smooth surface morphology, this makes it ideal for use in bead based assays as the microspheres do not get trapped in the membrane, allowing for efficient bead recovery. The filter plate is suggested no more than 350μL and no less than 1.2μm loading. |
96 well filter plate (960ul each well) |
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SD5006 | Bio Basic | 12UNIT | EUR 115.85 |
Black 96 Well Plate, 1EA |
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X025-1EA | Arbor Assays | 1EA | EUR 19 |
NanoECM®; 96 Well Plate |
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9601 | Neuromics | each | EUR 38.7 |
Adapter for 96 well plate |
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3510147 | Atto | 1unit | EUR 4422 |
NanoAligned®; 96 Well Plate |
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9602 | Neuromics | each | EUR 41.4 |
Aligned Nanofibers 96 Well Plate |
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3D00012-96 | Neuromics | 700 nm-PCLs | EUR 132 |
96 Well ELISA Plate, 8-Well, Detachable, High Binding, WhiteFrame & Black Well, |
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504261 | NEST Biotechnology | 50 pcs/cs | EUR 26.29 |
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, WhiteFrame & Black Well, 5/pk, 50/cs |
Streptavidin coated 96-well Plate |
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6523-5 | Biovision | each | EUR 392.4 |
Deep Well Plate 96 Well 2.0 ML - PK50 |
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AXYPDW20C | Scientific Laboratory Supplies | PK50 | EUR 221.4 |
96 Deep Well Plate Square Well V - PK50 |
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PCR0246 | Scientific Laboratory Supplies | PK50 | EUR 363.15 |
UNIFILTER PLATE 96 WELL 800 UL/WELL - PK25 |
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WHA77002803 | Scientific Laboratory Supplies | PK25 | EUR 1101.6 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
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P-DW-12-HC | CORNING | 5/pk | EUR 182.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
96 WELL PCR SEGMENTED PLATE |
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PCR-96-SG-C | CORNING | 10/pk | EUR 174 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & CLear Well, |
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504201 | NEST Biotechnology | 50 pcs/cs | EUR 38.5 |
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & CLear Well, 5/pk, 50/cs |
96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & White Well, |
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504271 | NEST Biotechnology | 50 pcs/cs | EUR 51.15 |
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & White Well, 5/pk, 50/cs |
Tissue Culture Plate, 96 Well |
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TCP20-96 | Bio Basic | 1 UNIT | EUR 64.18 |
Clear Coated 96 Well Plate, 1EA |
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C107-1EA | Arbor Assays | 1EA | EUR 162 |
Brand 96 Well Deep Well Plate-Stackable - PK24 |
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BR701354-24EA | Scientific Laboratory Supplies | PK24 | EUR 183.6 |
96-Well Protein Folding Plate |
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PFS096 | ProFoldin | kit | EUR 347.84 |
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins. |
BioMag® 96-Well Plate Separator |
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8MB4109S-1 | Polysciences Europe GmbH | 1magnet | EUR 955 |
Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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PRTA55-5P | Alpha Diagnostics | 1 PK | EUR 315.6 |
Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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PRTG65-5P | Alpha Diagnostics | 1 PK | EUR 315.6 |
DEEP WELL PLATE 96 WELL 2.0 ML STERIL - PK50 |
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AXYPDW20CS | Scientific Laboratory Supplies | PK50 | EUR 270 |
96 well PCR plate ns - PK50 |
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AXY2164 | Scientific Laboratory Supplies | PK50 | EUR 268.65 |
Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
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CT365 | U-CyTech | 5 | EUR 93.6 |
2 x Clear 96 well Plate, 2EA |
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X003-2EA | Arbor Assays | 2EA | EUR 13 |
5 x Clear 96 well Plate, 5EA |
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X003-5EA | Arbor Assays | 5EA | EUR 25 |
2 x Clear 96 well Plate, 2EA |
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X018-2EA | Arbor Assays | 2EA | EUR 13 |
4 x Clear 96 Well Plate, 4EA |
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X018-4EA | Arbor Assays | 4EA | EUR 31 |
96 Deep Well Plate Round Wells - PK50 |
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PCR0242 | Scientific Laboratory Supplies | PK50 | EUR 329.4 |
Plate 96 Well Immulon II - PK50 |
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3455 | Scientific Laboratory Supplies | PK50 | EUR 318.6 |
96 Well Roche Style PCR Plate |
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PCR1218 | Scientific Laboratory Supplies | PK50 | EUR 223.74 |
Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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PRTAG75-5P | Alpha Diagnostics | 1 PK | EUR 343.2 |
96 Deep Well Plate Square WellU - PK50 |
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PCR0256 | Scientific Laboratory Supplies | PK50 | EUR 261.9 |
Nunc Lid for 96 well plate - PK50 |
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264122 | Scientific Laboratory Supplies | PK50 | EUR 143.1 |
96 Well Skirted PCR Plate - PK50 |
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PCR1236 | Scientific Laboratory Supplies | PK50 | EUR 433.35 |
96 well filter plate (no filter) |
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SD5006-EMPTY | Bio Basic | 12UNIT | EUR 104.89 |
96 Deep Well Plate Square Wells - PK100 |
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PCR0240 | Scientific Laboratory Supplies | PK100 | EUR 378 |
96 Well Semi Skirted Plate - PK50 |
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PCR1216 | Scientific Laboratory Supplies | PK50 | EUR 229.5 |
Black Half Area 96 Well Plate, 1EA |
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X023-1EA | Arbor Assays | 1EA | EUR 25 |
Genorise® Feline CRP Polyclonal Antibody |
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GR195052 | Genorise Scientific | 100 µg | EUR 398 |
96 well DNA plate with membrane (960ul each well) |
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SD5007 | Bio Basic | 12UNIT | EUR 291.24 |
96 well RNA plate with membrane (960ul each well) |
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SD5009 | Bio Basic | 12UNIT | EUR 313.16 |
96 Well PCR Plate Clear Wells - PK50 |
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PCR0972 | Scientific Laboratory Supplies | PK50 | EUR 290.25 |
96-well plate Plasmid DNA Prep (mini) |
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TBS6012 | Tribioscience | 2x 96-well Plates | EUR 442 |
Random Access 96 Well Plate Skir - PK50 |
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PCR1412 | Scientific Laboratory Supplies | PK50 | EUR 338.85 |
96-Well Plate Viral DNA Miniprep Kit |
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VT92032 | Bio Basic | 2XPlates, 192prep | EUR 495.86 |
96-Well Plate Plasmid DNA Miniprep Kit |
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BS415 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate Plasmid DNA Miniprep Kit |
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BS4152 | Bio Basic | 2XPlates, 192prep | EUR 186.19 |
96-Well Plate DNA Cleanup Miniprep Kit |
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BS369 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate DNA Cleanup Miniprep Kit |
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BS3692 | Bio Basic | 2xPlates, 192prep | EUR 186.19 |
96 Well ELISA Plate, Undetachable, High Binding, Clear Frame & CLear Well, 5/pk, |
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514201 | NEST Biotechnology | 50 pcs/cs | EUR 30.36 |
Description: 96 Well ELISA Plate, Undetachable, High Binding, Clear Frame & CLear Well, 5/pk, 50/cs |
PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
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CT366 | U-CyTech | 5 | EUR 268.8 |
Falcon Microtest Black 96 Well Plate - PK32 |
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353376 | Scientific Laboratory Supplies | PK32 | EUR 244.35 |
96-Well Plate PCR Products Purification Kit |
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BS365 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate PCR Products Purification Kit |
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BS3652 | Bio Basic | 2xPlates, 192prep | EUR 186.19 |
Benchmark Beadblaster 96 Adapter for 96 Well Plate - PK2 |
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HOM3208 | Scientific Laboratory Supplies | PK2 | EUR 1923.75 |
96 WELL CLEAR PCR PLATE FOR ABI |
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PCR-96-AB-C | CORNING | 10/pk | EUR 200.4 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96 Well ABI Fast Plate Pcr - PK50 |
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PCR1200 | Scientific Laboratory Supplies | PK50 | EUR 290.25 |
96 Deep Well Plate Round Wells U - PK100 |
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PCR0228 | Scientific Laboratory Supplies | PK100 | EUR 244.35 |
96 Deep Well Plate Round Wells V - PK100 |
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PCR0230 | Scientific Laboratory Supplies | PK100 | EUR 244.35 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
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P-DW-12-HC-S | CORNING | 5/pk | EUR 248.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
96 Well ExoELISA plate (12x8 well strips, pack of 10 plates) |
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EXOEL-96P-1 | SBI | 10 Plates | EUR 255.6 |
Plate 96 Well Immulon 1 FB - PK50 |
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N3355 | Scientific Laboratory Supplies | PK50 | EUR 233.55 |
Nunc Lid for 96 Well Plate SH - PK100 |
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263339 | Scientific Laboratory Supplies | PK100 | EUR 126.9 |
Deep-Multiple Well Plate 96 wells, U ty |
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DM1-50NO | EWC Diagnostics | 1 unit | EUR 483.15 |
Description: Deep-Multiple Well Plate 96 wells, U ty |
Deep-Multiple Well Plate 96 wells, U ty |
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DM4-24NO | EWC Diagnostics | 1 unit | EUR 295.56 |
Description: Deep-Multiple Well Plate 96 wells, U ty |
Deep-Multiple Well Plate 96 wells, U ty |
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DM4-48NO | EWC Diagnostics | 1 unit | EUR 572.66 |
Description: Deep-Multiple Well Plate 96 wells, U ty |
Adapter for 96 well plate, pack of 2 |
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IPD9600-96 | Benchmark Scientific | each | EUR 1337.97 |
96 Well PCR Plate Divisible In 8 |
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PCR1062 | Scientific Laboratory Supplies | PK50 | EUR 154.44 |
Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate - EACH |
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HOM3232 | Scientific Laboratory Supplies | EACH | EUR 136.35 |
Block for 96 well plate, WSC-2620/30 |
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4002659 | Atto | 1unit | EUR 458.4 |
96 Well Roche Style Pcr Plate - PK50 |
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PCR1214 | Scientific Laboratory Supplies | PK50 | EUR 330.75 |
PCR Plate Non Skirted 96 Well - PK50 |
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MOL2364 | Scientific Laboratory Supplies | PK50 | EUR 348.3 |
Nori® Feline CRP POCT Systems-25 Tests |
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GR222008 | Genorise Scientific | 25 Tests | EUR 299 |
96 Well Roche Style Qpcr Plate - PK50 |
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PCR1210 | Scientific Laboratory Supplies | PK50 | EUR 318.6 |
PCR Plate Semi Skirted 96 Well - PK50 |
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MOL2366 | Scientific Laboratory Supplies | PK50 | EUR 338.85 |
Presto™ RNA 96 Well Binding Plate |
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96RBP005 | Geneaid Biotech | each | EUR 132 |
Presto™ RNA 96 Well Binding Plate |
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96RBP100 | Geneaid Biotech | each | EUR 2200 |
96 Well Lung Tumor Genomic DNA Plate |
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D8235152-1 | Biochain | 1 plate | EUR 377 |
96 Well Skirted Plate+ Q-Stick - PK50 |
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PCR1238 | Scientific Laboratory Supplies | PK50 | EUR 311.85 |
Presto™ gDNA 96 Well Binding Plate |
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96GBP005 | Geneaid Biotech | each | EUR 103 |
Presto™ gDNA 96 Well Binding Plate |
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96GBP100 | Geneaid Biotech | each | EUR 1854 |
Presto™ Plasmid 96 Well Filter Plate |
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96PFP005 | Geneaid Biotech | each | EUR 78.5 |
Presto™ Plasmid 96 Well Filter Plate |
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96PFP100 | Geneaid Biotech | each | EUR 1415 |
96 Well Colon Tumor Genomic DNA Plate |
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D8235090-1 | Biochain | 1 plate | EUR 377 |
S100 96-well PCR Plate Adapter - EACH |
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E5391070026 | Scientific Laboratory Supplies | EACH | EUR 426.6 |
S200 96-well PCR Plate Adapter - EACH |
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E5392070038 | Scientific Laboratory Supplies | EACH | EUR 426.6 |
96 Well PCR Plate Divisible In12 - PK50 |
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PCR1064 | Scientific Laboratory Supplies | PK50 | EUR 195.75 |
96 Well Semi-Skirted PCR Plate - PK50 |
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PCR1106 | Scientific Laboratory Supplies | PK50 | EUR 414.45 |
96-Well G-25 Gel Filtration Plate |
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96CG02 | Geneaid Biotech | each | EUR 250 |
96-Well G-25 Gel Filtration Plate |
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96CG04 | Geneaid Biotech | each | EUR 475 |
96-Well G-25 Gel Filtration Plate |
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96CG10 | Geneaid Biotech | each | EUR 1065 |
96-Well G-50 Gel Filtration Plate |
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CGP02 | Geneaid Biotech | each | EUR 250 |
96-Well G-50 Gel Filtration Plate |
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CGP04 | Geneaid Biotech | each | EUR 589.6 |
96-Well G-50 Gel Filtration Plate |
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CGP10 | Geneaid Biotech | each | EUR 1382 |
Presto™ Plasmid 96 Well Binding Plate |
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96PBP005 | Geneaid Biotech | each | EUR 78.5 |
Presto™ Plasmid 96 Well Binding Plate |
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96PBP100 | Geneaid Biotech | each | EUR 1415 |
96 Well Breast Tumor Genomic DNA Plate |
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D8235086-1 | Biochain | 1 plate | EUR 377 |
96 Well Solid Bottom Plate Black - PK100 |
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PCR0354 | Scientific Laboratory Supplies | PK100 | EUR 475.2 |
96 Well Solid Bottom Plate White - PK100 |
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PCR0360 | Scientific Laboratory Supplies | PK100 | EUR 437.4 |
96 Well Impact MicroBatch Plate, 80/CSE |
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M-673101 | MiTeGen | 80 PLATES | EUR 808 |
Description: 96 Well Impact MicroBatch Plate, 80/CSE |
96 WELL PLATE; SQUARE; U BASE; 1.6ML; PP; 96/PK - PK96 |
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60180-P155 | Scientific Laboratory Supplies | PK96 | EUR 631.8 |
Olympus 96-Well PCR Plate, FAST-type |
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24-310 | Genesee Scientific | 25 Plates/Unit | EUR 57.82 |
Description: Low Profile, Natural |
Olympus 96-Well PCR Plate, FAST-type |
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24-310W | Genesee Scientific | 10 Plates/Unit | EUR 29.37 |
Description: Low Profile, White |
96 Well Stomach Tumor Genomic DNA Plate |
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D8235248-1 | Biochain | 1 plate | EUR 377 |
Mouse anti-ACTH Clear Coated 96 Well Plate |
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C271-1EA | Arbor Assays | 1EA | EUR 150 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92031 | Bio Basic | 2Plates, 192prep | EUR 210.85 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92032 | Bio Basic | 5Plates, 480prep | EUR 389.9 |
96 Well Plate Yeast Genomic DNA Miniprep Kit |
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BS8357 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate) |
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M-R-1008649 | MiTeGen | 98 X 1.7 ml | EUR 316 |
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate) |
96-Well Plate Plant Genomic DNA Miniprep Kit |
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BS8361 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
CORNING® BIOCOAT™ GELATIN 96 WELL CLEAR FLAT BOTTOM ASSAY PLATE, WITH LID, NONSTERILE, 5/PACK, 50/CASE |
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356689 | CORNING | 5/pk | EUR 800.4 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
Olympus 96-Well PCR Plate, Non-Skirted |
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24-300 | Genesee Scientific | 25 Plates/Unit | EUR 57.13 |
Description: Ultra Thin Wall, Natural |
Genorise® Biotinylated Feline CRP Polyclonal Antibody |
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GR196052 | Genorise Scientific | 100 µg | EUR 398 |
96 Well Plate RNA Cleanup and Concentration Kit |
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BRC1251 | Bio Basic | 2xPlates, 192prep | EUR 300.37 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS437 | Bio Basic | 5XPlates, 480prep | EUR 435.58 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS4372 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
Olympus 96-Well PCR Plate, Semi-Skirted |
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24-301 | Genesee Scientific | 25 Plates/Unit | EUR 57.82 |
Description: Raised Rim, Natural |
Olympus 96-Well PCR Plate, Full-Skirted |
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24-302 | Genesee Scientific | 25 Plates/Unit | EUR 60.31 |
Description: Ultra Thin Wall, Natural |
Olympus 96-Well PCR Plate, Full-Skirted |
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24-302W | Genesee Scientific | 10 Plates/Unit | EUR 27.75 |
Description: Ultra Thin Wall, White |
Olympus 96-Well PCR Plate, Semi-Skirted |
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27-108 | Genesee Scientific | 10 Plates/Unit | EUR 23.71 |
Description: Flat Rim, Natural |
Olympus 96-Well PCR Plate, Semi-Skirted |
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27-408 | Genesee Scientific | 10 Plates/Unit | EUR 23.76 |
Description: Straight-Sided, Natural |
Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
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M-R-1008647 | MiTeGen | 96 X 1.7 ml | EUR 316 |
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
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M-R-1008648 | MiTeGen | 97 X 1.7 ml | EUR 316 |
Description: Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
96-well Plasmid DNA Extraction Kit (1 plate) |
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FAPWE-96001 | Favorgen | 1 prep | EUR 175.2 |
96-well Plasmid DNA Extraction Kit (1 plate) |
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FAPWE_96001 | Favorgen | 1 kit | EUR 36 |
96 WELL PLATE; SQUARE; 2ML; PP; 1/PK - EACH |
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60180-P403 | Scientific Laboratory Supplies | EACH | EUR 21.6 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1292 | Bio Basic | 2XPlates, 192prep | EUR 210.85 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1295 | Bio Basic | 5XPlates, 480prep | EUR 389.9 |
CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
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354730 | CORNING | 5/pk | EUR 102 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
2 x Black Half Area 96 Well Plate, 2EA |
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X037-2EA | Arbor Assays | 2EA | EUR 37 |
EVA Cap Mat for 96 well deep well plate, DNase/RNase Free |
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IPD1196-CM | Benchmark Scientific | each | EUR 92.08 |