Immunofluorescence Workflow

Tissue preparation

 VECTABOND adhesive for tissue sections
 ImmPrint histological pen
 ImmEdge hydrophobic barrier pen

Antigen unmasking

 Antigen unmasking solutions, based on citrate or Tris

Extinguishing / blocking

 TrueVIEW autofluorescence kit **
 TrueVIEW kit for autofluorescence quenching with DAPI **
 Avidin / biotin blocking kit
 Streptavidin / biotin blocking kit
 Normal serum
 Animal-Free Blocker® and thinner
 BSA
⯈ Casein solution
 MOM (mouse to mouse) blocking agent
 Carbo-Free ™ blockers
 BLOXALL solution for blocking endogenous HRP / AP

Primary antibodies / lectins *

 Biotinylated lectins
 Fluorochrome conjugated lectins
 Unconjugated lectins

Secondary

antibody

 VectaFluor DyLight RTU secondary antibody
 VectaFluor Duet IF kits for double marking
 MOM (Mouse to Mouse) immunodetection kits
 Biotinylated secondary antibodies
 Fluorochrome-conjugated secondary antibodies
 Unconjugated secondary antibodies

Tertiary reagent

 VectaFluor Excel DyLight amplified fluorescent staining systems
 Fluorochrome conjugated to avidin or streptavidin
 Biotinylated anti-avidin amplification reagent
 Biotinylated anti-streptavidin amplification reagent

Contrast staining /

assembly

⯈ VECTASHIELD Antifade mounting medium
⯈ VECTASHIELD Antifade mounting medium with DAPI
⯈ VECTASHIELD Antifade mounting medium with propidium iodide (PI)
 VECTASHIELD HardSet Antifade mounting medium
 VECTASHIELD HardSet Antifade mounting medium with DAPI
 VECTASHIELD HardSet Antifade mounting medium with TRITC-phaloidin
ECT VECTASHIELD Vibrance Antifade mounting medium
 VECTASHIELD Vibrance Antifade mounting medium with DAPI