Immunofluorescence Workflow

Tissue preparation

 VECTABOND adhesive for tissue sections
 ImmPrint histological pen
 ImmEdge hydrophobic barrier pen

Antigen unmasking

 Antigen unmasking solutions, based on citrate or Tris

Extinguishing / blocking

 TrueVIEW autofluorescence kit **
 TrueVIEW kit for autofluorescence quenching with DAPI **
 Avidin / biotin blocking kit
 Streptavidin / biotin blocking kit
 Normal serum
 Animal-Free Blocker® and thinner
⯈ Casein solution
 MOM (mouse to mouse) blocking agent
 Carbo-Free ™ blockers
 BLOXALL solution for blocking endogenous HRP / AP

Primary antibodies / lectins *

 Biotinylated lectins
 Fluorochrome conjugated lectins
 Unconjugated lectins



 VectaFluor DyLight RTU secondary antibody
 VectaFluor Duet IF kits for double marking
 MOM (Mouse to Mouse) immunodetection kits
 Biotinylated secondary antibodies
 Fluorochrome-conjugated secondary antibodies
 Unconjugated secondary antibodies

Tertiary reagent

 VectaFluor Excel DyLight amplified fluorescent staining systems
 Fluorochrome conjugated to avidin or streptavidin
 Biotinylated anti-avidin amplification reagent
 Biotinylated anti-streptavidin amplification reagent

Contrast staining /


⯈ VECTASHIELD Antifade mounting medium
⯈ VECTASHIELD Antifade mounting medium with DAPI
⯈ VECTASHIELD Antifade mounting medium with propidium iodide (PI)
 VECTASHIELD HardSet Antifade mounting medium
 VECTASHIELD HardSet Antifade mounting medium with DAPI
 VECTASHIELD HardSet Antifade mounting medium with TRITC-phaloidin
ECT VECTASHIELD Vibrance Antifade mounting medium
 VECTASHIELD Vibrance Antifade mounting medium with DAPI