Immunofluorescence Workflow

Tissue preparation

⯈ VECTABOND adhesive for tissue sections
⯈ ImmPrint histological pen
⯈ ImmEdge hydrophobic barrier pen

Antigen unmasking

⯈ Antigen unmasking solutions, based on citrate or Tris

Extinguishing / blocking

⯈ TrueVIEW autofluorescence kit **
⯈ TrueVIEW kit for autofluorescence quenching with DAPI **
⯈ Avidin / biotin blocking kit
⯈ Streptavidin / biotin blocking kit
⯈ Normal serum
⯈ Animal-Free Blocker® and thinner
⯈ BSA
⯈ Casein solution
⯈ MOM (mouse to mouse) blocking agent
⯈ Carbo-Free ™ blockers
⯈ BLOXALL solution for blocking endogenous HRP / AP

Primary antibodies / lectins *

⯈ Biotinylated lectins
⯈ Fluorochrome conjugated lectins
⯈ Unconjugated lectins

Secondary

antibody

⯈ VectaFluor DyLight RTU secondary antibody
⯈ VectaFluor Duet IF kits for double marking
⯈ MOM (Mouse to Mouse) immunodetection kits
⯈ Biotinylated secondary antibodies
⯈ Fluorochrome-conjugated secondary antibodies
⯈ Unconjugated secondary antibodies

Tertiary reagent

⯈ VectaFluor Excel DyLight amplified fluorescent staining systems
⯈ Fluorochrome conjugated to avidin or streptavidin
⯈ Biotinylated anti-avidin amplification reagent
⯈ Biotinylated anti-streptavidin amplification reagent

Contrast staining /

assembly

⯈ VECTASHIELD Antifade mounting medium
⯈ VECTASHIELD Antifade mounting medium with DAPI
⯈ VECTASHIELD Antifade mounting medium with propidium iodide (PI)
⯈ VECTASHIELD HardSet Antifade mounting medium
⯈ VECTASHIELD HardSet Antifade mounting medium with DAPI
⯈ VECTASHIELD HardSet Antifade mounting medium with TRITC-phaloidin
ECT VECTASHIELD Vibrance Antifade mounting medium
⯈ VECTASHIELD Vibrance Antifade mounting medium with DAPI