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Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells

Posted by Darren Marshall on in Antibodies, Assay Kits, Biology Cells, Blog, cDNA, Clia Kits, Culture Cells, Devices, DNA, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, Recombinant Proteins, Ria Kits, RNA, Simultaneous Enhancement of Near-Infrared Emission and Dye Photodegradation in a Racemic Aspartic Acid Compound via Metal-Ion Modification., Synthesis of Highly Functionalized 2-Pyranone from Silyl Ketene., Thermodynamic and kinetic studies of H2 and N2 binding to bimetallic nickel-group 13 complexes and neutron structure of a Ni(η2-H2) adduct., Western Blot
Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells

Protein evaluation in bone marrow samples from sufferers with a number of myeloma (MM) has been restricted by the low focus of proteins obtained after CD138+ cell choice. A novel method based mostly on capillary nano-immunoassay may make it doable to quantify dozens of proteins from every CD138+ purified MM pattern in an automatic method. Up to now, the information of protein degree in these cells was restricted as a result of a comparatively small amount of pattern is on the market after the diagnostic process. Moreover, the pattern usually is required for nucleic acids evaluation.

We have developed the process for acquiring proteins from bone marrow samples preserved in RLT+ buffer, and now we have efficiently utilized this method for the quantification of proteins within the setting of sufferers with MM. Proteins are extracted from RLT+ buffer, the content material is quantified by whole protein assay with WES machine and eventually, the actual protein expression degree is evaluated utilizing particular antibodies by capillary nano-immunoassay with WES machine. The current protocol permits us to quantify many proteins from a restricted quantity of pattern, with out shedding the chance to acquire nucleic acids on the identical time. Proteins are quantified robotically in an assay with a low chance of human errors, which makes it a useful gizmo for biomarkers improvement.

Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization

Numerous experimental approaches exist to check interactions between two subunits of a big macromolecular complicated. However, most strategies don’t present spatial and temporal details about binding, that are crucial for dissecting the mechanism of meeting of nanosized complexes in vivo. While latest advances in super-resolution microscopy methods have supplied insights into organic buildings past the diffraction restrict, most require in depth experience and/or particular pattern preparation, and it’s a problem to increase past binary, two colour experiments.

Using HyVolution, a super-resolution method that mixes confocal microscopy at sub-airy unit pinhole sizes with computational deconvolution, we achieved 140 nm decision in each dwell and stuck samples with three colours, together with two fluorescent proteins (mTurquoise2 and GFP) with important spectral overlap that had been distinguished by means of shifting the excitation wavelength away from widespread wavelengths. By combining HyVolution super-resolution fluorescence microscopy with bimolecular fluorescence complementation (SRM-BiFC), we describe a brand new assay succesful of visualizing protein-protein interactions in vivo at sub-diffraction decision. This methodology was used to enhance our understanding of the ordered meeting of the Saccharomyces cerevisiae spindle pole physique (SPB), a ~1 giga-Dalton heteromeric protein complicated fashioned from 18 structural parts current in a number of copies. We suggest that SRM-BiFC is a robust device for examination of direct interactions between protein complicated subunits at sub-diffraction decision in dwell cells.

Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells

Interaction between Borrelia miyamotoi variable main proteins Vlp15/16 and Vlp18 with plasminogen and complement

Borrelia miyamotoi, a relapsing fever spirochete transmitted by Ixodid ticks causes B. miyamotoi illness (BMD). To evade the human host´s immune response, relapsing fever borreliae, together with B. miyamotoi, produce distinct variable main proteins. Here, we investigated Vsp1, Vlp15/16, and Vlp18 all of that are at present being evaluated as antigens for the serodiagnosis of BMD. Comparative analyses recognized Vlp15/16 however not Vsp1 and Vlp18 as a plasminogen-interacting protein of B. miyamotoi. Furthermore, Vlp15/16 certain plasminogen in a dose-dependent vogue with excessive affinity.
Binding of plasminogen to Vlp15/16 was considerably inhibited by the lysine analog tranexamic acid suggesting that the protein-protein interplay is mediated by lysine residues. By distinction, ionic energy didn’t impact binding of plasminogen to Vlp15/16. Of relevance, plasminogen certain to the borrelial protein cleaved the chromogenic substrate S-2251 upon conversion by urokinase-type plasminogen activator (uPa), demonstrating it retained its physiological exercise. Interestingly, additional analyses revealed a complement inhibitory exercise of Vlp15/16 and Vlp18 on the choice pathway by a Factor H-independent mechanism. More importantly, each borrelial proteins shield serum delicate Borrelia garinii cells from complement-mediated lysis suggesting a number of roles of these two variable main proteins in immune evasion of B. miyamotoi.

Mouse Anti Feline Leukaemia Virus P27 Monoclonal Antibody
DMABT-51281MF 0.25 mg
EUR 767

Goat Anti Feline Leukaemia Virus P27 Polyclonal Antibody
DPBT-66847GF 1 ml
EUR 861

Feline Leukemia Virus p27 antibody
10-1520 100 ug
EUR 337
Description: Mouse monoclonal Feline Leukemia Virus p27 antibody

Feline Leukemia virus p27 antibody
10-F05A 250 ug
EUR 308
Description: Mouse monoclonal Feline Leukemia virus antibody

Feline Leukemia Virus p27 antibody
10-F05C 250 ug
EUR 217
Description: Mouse monoclonal Feline Leukemia Virus p27 antibody

Feline Leukemia Virus p27 antibody
20-FG32 1 mg
EUR 128
Description: Goat polyclonal Feline Leukemia Virus p27 antibody

Feline Leukaemia Virus gp70 (FeLV gp70) Antibody
abx413483-025mg 0.25 mg
EUR 592
  • Shipped within 1 week.

Feline Leukaemia Virus p15E (FeLV p15E) Antibody
abx414340-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

Feline Leukemia Virus p27 protein
30-1928 100 ug
EUR 457
Description: Recombinant FeLV p27 protein with his tag

Feline Leukemia Virus p27 antibody (HRP)
60-5006 1 ml
EUR 409
Description: Goat polyclonal Feline Leukemia Virus p27 antibody (HRP)

Feline Leukemia Virus p27 antibody (biotin)
60-5007 1 ml
EUR 378
Description: Goat polyclonal Feline Leukemia Virus p27 antibody (biotin)

Mouse Anti Feline Leukaemia Virus P15e Monoclonal Antibody
DMABT-51278MF 0.25 mg
EUR 767

Mouse antibody for Feline Leukemia virus FeLv p27
7218 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Mouse antibody for Feline Leukemia virus FeLv p27
7226 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Mouse antibody for Feline Leukemia virus FeLv p27
7228 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Feline Leukemia virus antibody
10C-CR1297M1 100 ug
EUR 247
Description: Mouse monoclonal Feline Leukemia virus antibody

Feline Leukemia Virus gp70 antibody
10-F07A 250 ug
EUR 327
Description: Mouse monoclonal Feline Leukemia Virus gp70 antibody

Feline Leukemia Virus gp70 antibody
10-F07B 250 ug
EUR 327
Description: Mouse monoclonal Feline Leukemia Virus gp70 antibody

Feline Panleukopenia Virus PCR kit
PCR-V171-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline Panleukopenia Virus

Feline Panleukopenia Virus PCR kit
PCR-V171-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline Panleukopenia Virus

Feline immunodeficiency virus PCR kit
PCR-V175-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline immunodeficiency virus

Feline immunodeficiency virus PCR kit
PCR-V175-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline immunodeficiency virus

Feline leukemia virus PCR kit
PCR-V176-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline leukemia virus

Feline leukemia virus PCR kit
PCR-V176-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline leukemia virus

Goat polyclonal antibody for Feline Leukemia virus
274 1 ml
EUR 321.88
Description: This is HRP conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus
7221 1 ml
EUR 283.81
Description: This is goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus
7223 1 ml
EUR 300.13
Description: This is FITC conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus
7227 1 ml
EUR 300.13
Description: This is Biotin conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Feline Herpes Virus Type 1 (FHV1) Antibody
abx414268-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

Cat Leukemia Virus p27 Protein
20-abx260224
  • EUR 885.00
  • EUR 342.00
  • EUR 1609.00
  • 0.5 mg
  • 100 ug
  • 1 mg
  • Shipped within 5-10 working days.

Recombinant Feline Immunodeficiency Virus Core Antigen
DAG552 100ug
EUR 949

Native Feline Infectious Peritonitis Virus antigen
DAGA-3038 1mg
EUR 1040

Feline Immunodef. virus core recombinant antigen
8963 100 ug
EUR 561.13
Description: This is Feline Immunodef. virus core recombinant antigen for ELISA,WB.

Feline Panleukopenia Virus RT PCR kit
RTq-V171-100D 100T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline Panleukopenia Virus .

Feline Panleukopenia Virus RT PCR kit
RTq-V171-150D 150T
EUR 701
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline Panleukopenia Virus .

Feline Panleukopenia Virus RT PCR kit
RTq-V171-50D 50T
EUR 532.8
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline Panleukopenia Virus .

Feline immunodeficiency virus RT PCR kit
RTq-V175-100R 100T
EUR 776.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline immunodeficiency virus .

Feline immunodeficiency virus RT PCR kit
RTq-V175-150R 150T
EUR 877.9
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline immunodeficiency virus .

Feline immunodeficiency virus RT PCR kit
RTq-V175-50R 50T
EUR 631.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline immunodeficiency virus .

Feline leukemia virus RT PCR kit
RTq-V176-100R 100T
EUR 776.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline leukemia virus .

Feline leukemia virus RT PCR kit
RTq-V176-150R 150T
EUR 877.9
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline leukemia virus .

Feline leukemia virus RT PCR kit
RTq-V176-50R 50T
EUR 631.4
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Feline leukemia virus .

Mouse antibody for Feline Leukemia virus FeLv gp70
7217 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv gp70 for WB, ELISA.

Mouse Anti-Feline Leukemia Virus (FeLV) monoclonal antibody
CABT-BL8814 1.0 mg
EUR 892

Mouse Anti-Feline Leukemia Virus (FeLV) monoclonal antibody
CABT-BL8815 1.0 mg
EUR 892

Feline Immunodeficiency Virus p24 Antigen (FIV p24) Antibody
abx411336-02mg 0.2 mg
EUR 592
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Feline Immunodeficiency Virus gp41 Antigen (FIV gp41) Antibody
abx411337-02mg 0.2 mg
EUR 592
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Feline Immunodeficiency Virus p24 Antigen (FIV p24) Antibody
abx412988-2ml 2 ml
EUR 606
  • Shipped within 1 week.

Feline Immunodeficiency Virus (F1a), RNA Aptamer, unlabeled
AR-230-U Custom Ask for price

Feline Panleukopenia Virus One-Step PCR kit
Oneq-V171-100D 100T
EUR 747.4
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline Panleukopenia Virus.

Feline Panleukopenia Virus One-Step PCR kit
Oneq-V171-150D 150T
EUR 838.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline Panleukopenia Virus.

Feline Panleukopenia Virus One-Step PCR kit
Oneq-V171-50D 50T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline Panleukopenia Virus.

Feline immunodeficiency virus One-Step PCR kit
Oneq-V175-100R 100T
EUR 933
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline immunodeficiency virus.

Feline immunodeficiency virus One-Step PCR kit
Oneq-V175-150R 150T
EUR 1060.6
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline immunodeficiency virus.

Feline immunodeficiency virus One-Step PCR kit
Oneq-V175-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline immunodeficiency virus.

Feline leukemia virus One-Step PCR kit
Oneq-V176-100R 100T
EUR 933
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline leukemia virus.

Feline leukemia virus One-Step PCR kit
Oneq-V176-150R 150T
EUR 1060.6
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline leukemia virus.

Feline leukemia virus One-Step PCR kit
Oneq-V176-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Feline leukemia virus.

Feline Immunodeficiency Virus gp95 Envelope Antigen (FIV gp95) Antibody
abx411335-02mg 0.2 mg
EUR 592
  • Shipped within 1 week.

Feline Immunodeficiency Virus gp95 Envelope Antigen (FIV gp95) Antibody
abx414342-025mg 0.25 mg
EUR 592
  • Shipped within 1 week.

P27 antibody
70R-16333 50 ul
EUR 435
Description: Rabbit polyclonal P27 antibody

p27 antibody
70-PR78 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal p27 antibody

p27 antibody
70R-13747 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal p27 antibody

p27 antibody
20-PR78 100 µL
EUR 755
Description: Rabbit polyclonal p27 antibody

p27 Antibody
DF6090 200ul
EUR 304
Description: p27 Antibody detects endogenous levels of total p27.

p27 Antibody
abx149167-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

p27 Antibody
ABD6090 100 ug
EUR 438

Feline FeLV antibody
10-2328 100 ug
EUR 325
Description: Mouse monoclonal Feline FeLV antibody for lateral flow

Feline FeLV antibody
10-2329 100 ug
EUR 325
Description: Mouse monoclonal Feline FeLV antibody for lateral flow

Feline Immunodeficiency Virus p24 gag Antigen (FIV p24 gag) Antibody
abx413943-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody
20-abx006544
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVC2) Antibody
abx034469-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVC2) Antibody
abx034469-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody
20-abx322439
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody
20-abx322440
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Feline Calicivirus (FCV) Antibody
abx413481-025mg 0.25 mg
EUR 676
  • Shipped within 1 week.

p27 Polyclonal Antibody
41299-100ul 100ul
EUR 252

p27 Polyclonal Antibody
41299-50ul 50ul
EUR 187

p27 Polyclonal Antibody
41300-100ul 100ul
EUR 252

p27 Polyclonal Antibody
41300-50ul 50ul
EUR 187

p27 Kip1 antibody
70R-49527 100 ul
EUR 287
Description: Purified Polyclonal p27 Kip1 antibody

p27 Kip1 antibody
70R-49528 100 ul
EUR 244
Description: Purified Polyclonal p27 Kip1 antibody

p27 Kip1 Antibody
20-abx009454
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

p27 Kip1 Antibody
20-abx123451
  • EUR 592.00
  • EUR 857.00
  • EUR 411.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

p27 Kip1 Antibody
20-abx121943
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

p27 Kip1 Antibody
AF6324 200ul
EUR 304
Description: p27 Kip1 Antibody detects endogenous levels of total p27 Kip1.

P27; KIP1 Antibody
abx236068-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

p27 Polyclonal Antibody
ABP53452-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T157
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human. This p27 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T157

p27 Polyclonal Antibody
ABP53452-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T157
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human. This p27 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T157

p27 Polyclonal Antibody
ABP53452-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T157
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human. This p27 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T157

p27 Kip1 Antibody
ABF6324 100 ug
EUR 438

p27 Polyclonal Antibody
ABP52103-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T187
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human, Mouse, Rat. This p27 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T187

p27 Polyclonal Antibody
ABP52103-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T187
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human, Mouse, Rat. This p27 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T187

p27 Polyclonal Antibody
ABP52103-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T187
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human, Mouse, Rat. This p27 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T187

p27 Polyclonal Antibody
ABP52104-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T198
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human. This p27 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T198

p27 Polyclonal Antibody
ABP52104-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T198
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human. This p27 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T198

p27 Polyclonal Antibody
ABP52104-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of T198
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human. This p27 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of T198

p27 Polyclonal Antibody
ABP52105-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human p27 around the non-phosphorylation site of S10
  • Applications tips:
Description: A polyclonal antibody for detection of p27 from Human, Mouse, Rat. This p27 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p27 around the non-phosphorylation site of S10
The objective of cryoEM is to find out the buildings of biomolecules from electron micrographs. In many instances the processing is simple and will be dealt with with routine protocols. In different instances, the properties and conduct of the specimen require adaptions to correctly interpret the info. Here I describe the protocols for analyzing the upper order assemblies of the retinal adhesion protein, retinoschisin (RS1), utilizing the Bsoft bundle. The protocols for micrograph preprocessing, 2D classification and 3D alignment and reconstruction observe the same old patterns for the bulk of cryoEM specimens. The interpretation of the outcomes is particular to the branched community of RS1 filaments. The 2D class averages are used to find out the relative positions of the RS1 molecules, thus defining the interacting interfaces within the community. The main interface of the linear filament is then additional examined by reconstructing the “unit cell” and becoming the molecular fashions.