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Collaborative Computational Project No. 14

Collaborative Computational Project No. 14

Camel Mab stabilisation of Proteins for X-rtay christal structure detection

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gelsemium

Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation

Posted by Darren Marshall on May 9, 2021 in Antibodies, Assay Kits, Biology Cells, Blog, cDNA, Clia Kits, Culture Cells, Devices, DNA, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, Recombinant Proteins, Ria Kits, RNA, Simultaneous Enhancement of Near-Infrared Emission and Dye Photodegradation in a Racemic Aspartic Acid Compound via Metal-Ion Modification., Synthesis of Highly Functionalized 2-Pyranone from Silyl Ketene., Thermodynamic and kinetic studies of H2 and N2 binding to bimetallic nickel-group 13 complexes and neutron structure of a Ni(η2-H2) adduct., Western Blot
Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation

Protein-protein interactions represent the molecular foundations of nearly all organic processes. Co-immunoprecipitation (CoIP) experiments are most likely essentially the most broadly used technique to probe each heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that a number of nuclear proteins akin to transcription components are spatially distributed into native high-concentration clusters in mammalian cells, suggesting that many nuclear proteins self-interact. These observations have additional underscored the necessity for orthogonal biochemical approaches for testing if self-association happens, and if that’s the case, what the mechanisms are. Here, we describe a CoIP protocol particularly optimized to check self-association of endogenously tagged nuclear proteins (self-CoIP), and to judge the position of nucleic acids in such self-interaction. This protocol has confirmed dependable and sturdy in our fingers, and it may be used to check each homotypic and heterotypic (CoIP) protein-protein interactions.

The ASR protein household has been found thirty years in the past in lots of plant species and is concerned within the tolerance of numerous abiotic stresses akin to dehydration, salinity and warmth. Despite its significance, nothing is thought concerning the conserved ABA-Water Deficit Stress Domain (ABA-WDS) of the ASR gene household. In this examine, we characterised two ABA-WDS domains, remoted from durum wheat (TtABA-WDS) and barley (HvABA-WDS). Bioinformatics evaluation exhibits that they’re each persistently predicted to be intrinsically disordered. Hydrodynamic and round dichroism evaluation point out that each domains are largely disordered however belong to totally different structural courses, with HvABA-WDS and TtABA-WDS adopting a PreMolten Globule-like (PMG-like) and a Random Coil-like (RC-like) conformation, respectively. In the presence of the secondary construction stabilizer trifluoroethanol (TFE) or of rising glycerol concentrations, which mimics dehydration, the 2 domains purchase an α-helical construction. Interestingly, each domains are capable of stop heat- and dehydration-induced inactivation of the enzyme lactate dehydrogenase (LDH).

Follistatin secretion is enhanced by protein, however not glucose or fats ingestion in overweight individuals independently of earlier gastric bypass surgical procedure

Follistatin is secreted from the liver and is concerned within the regulation of muscle mass and insulin sensitivity through inhibition of activin A in people. The secretion of follistatin appears to be stimulated by glucagon and inhibited by insulin, however solely restricted information on the postprandial regulation of follistatin exists. Moreover, outcomes on postoperative modifications after Roux-en-Y gastric bypass (RYGB) are conflicting with experiences of elevated, unaltered and lowered fasting concentrations of follistatin. In this examine, we investigated postprandial follistatin and activin A concentrations after consumption of isocaloric quantities of protein, fats or glucose in overweight topics with and with out earlier RYGB to discover the regulation of follistatin by the person macronutrients. Protein consumption enhanced follistatin concentrations equally within the two teams, whereas glucose and fats ingestion didn’t change postprandial follistatin concentrations. Concentrations of activin A had been decrease after protein consumption in contrast with glucose consumption in RYGB. Glucagon concentrations had been additionally notably enhanced by protein consumption and tended to correlate with follistatin in RYGB. In conclusion, we demonstrated that protein consumption, however not glucose or fats, is a powerful stimulus for follistatin secretion in overweight topics and that this regulation is maintained after RYGB surgical procedure.
Repressor element-1 (RE-1) or neural restrictive silencer aspect (NRSE) sure with a zinc finger transcription repressor, RE-1 silencing transcription issue (REST, often known as neural restrictive silencer issue, NRSF) has been recognized as a basic repressor aspect in lots of genes, together with neuronal genes. Genes regulated by REST/NRSF regulate multifaceted neuronal phenotypes, and their defects within the equipment trigger neuropathies, issues of neuron exercise), autism and so forth. In REST repressions, the N-terminal repressor area recruits Sin3B through its paired amphipathic helix 1 (PAH1) area, which performs an vital position as a scaffold for histone deacetylase 1 and a couple of. This equipment has a essential position in sustaining neuronal robustness. In this examine, with a view to set up protein-protein interplay assays mimicking a binding floor between Sin3B and REST, we chosen vital amino acids from structural data of the PAH1/REST complicated after which tried to reconstitute it utilizing recombinant quick peptides derived from PAH1/REST.
Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation

The DEAD-box protein household of RNA helicases: sentinels for a myriad of mobile capabilities with rising roles in tumorigenesis

DEAD-box RNA helicases comprise a household inside helicase superfamily 2 and make up the biggest group of RNA helicases. They are a profoundly conserved household of RNA-binding proteins, carrying a generic Asp-Glu-Ala-Asp (D-E-A-D) motif that provides the household its identify. Members of the DEAD-box household of RNA helicases are engaged in all sides of RNA metabolism from biogenesis to decay. DEAD-box proteins ordinarily perform as constituents of monumental multi-protein complexes and it’s believed that interactions with different parts within the complexes could be answerable for the assorted capacities ascribed to those proteins. Therefore, their precise perform might be impacted by their interacting companions and to be profoundly context dependent.
This could give a clarification to the often inconsistent experiences proposing that DEAD-box proteins have each pro- and anti-proliferative capabilities in most cancers. There is rising proof that DEAD-box household of RNA helicases play pivotal capabilities in numerous mobile processes and in quite a few circumstances have been embroiled in mobile proliferation and/or neoplastic transformation. In numerous malignancy sorts, DEAD-box RNA helicases have been reported to own pro-proliferation and even oncogenic roles in addition to anti-proliferative or tumor suppressor capabilities.

Black 96 Well Plate, 1EA

X025-1EA Arbor Assays 1EA
EUR 73

Adapter for 96 well plate

3510147 Atto 1unit
EUR 5581

96 well filter plate (960ul each well)

SD5006 Bio Basic 12UNIT
EUR 96.54

96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C CORNING 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC CORNING 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Clear Coated 96 Well Plate, 1EA

C107-1EA Arbor Assays 1EA
EUR 189

96 well filter plate (no filter)

SD5006-EMPTY Bio Basic 12UNIT
EUR 87.41

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE

PCR-96-HS-C CORNING 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.

CP-AXYGEM-96-50 CORNING 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

96 well DNA plate with membrane (960ul each well)

SD5007 Bio Basic 12UNIT
EUR 242.7

96 well RNA plate with membrane (960ul each well)

SD5009 Bio Basic 12UNIT
EUR 260.97

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 Biochain 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 Biochain 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 Biochain 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 Biochain 1 plate
EUR 316

2 x Clear 96 well Plate, 2EA

X003-2EA Arbor Assays 2EA
EUR 65

5 x Clear 96 well Plate, 5EA

X003-5EA Arbor Assays 5EA
EUR 80

2 x Clear 96 well Plate, 2EA

X018-2EA Arbor Assays 2EA
EUR 65

4 x Clear 96 Well Plate, 4EA

X018-4EA Arbor Assays 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA

X023-1EA Arbor Assays 1EA
EUR 80

96-Well Plate PCR Products Purification Kit

BS365 Bio Basic 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit

BS3652 Bio Basic 2xPlates, 192prep
EUR 155.16

96-Well Plate DNA Cleanup Miniprep Kit

BS369 Bio Basic 5XPlates, 480prep
EUR 250.31

96-Well Plate DNA Cleanup Miniprep Kit

BS3692 Bio Basic 2xPlates, 192prep
EUR 155.16

96-Well Plate Plasmid DNA Miniprep Kit

BS415 Bio Basic 5XPlates, 480prep
EUR 250.31

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 Bio Basic 2XPlates, 192prep
EUR 155.16

96-Well Plate Viral DNA Miniprep Kit

VT92032 Bio Basic 2XPlates, 192prep
EUR 413.22

Polystyrene-bottomed 96-well plate + lid (ELISPOT)

CT365 U-CyTech 5
EUR 78

Radius 96-Well Cell Migration Assay

CBA-126 Cell Biolabs 96 assays
EUR 572
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 Cell Biolabs 5 x 96 assays
EUR 2248
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay

CBA-130 Cell Biolabs 96 assays
EUR 722
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 Cell Biolabs 5 x 96 assays
EUR 2886
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224 Cell Biolabs 96 assays
EUR 722
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224-5 Cell Biolabs 5 x 96 assays
EUR 2938
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

OxiSelect 96-Well Comet Assay Kit

STA-355 Cell Biolabs 96 assays
EUR 427
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit

STA-355-5 Cell Biolabs 5 x 96 assays
EUR 1633
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide

STA-356 Cell Biolabs 1 slide
EUR 183
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide

STA-356-5 Cell Biolabs 5 slides
EUR 519
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit

STA-416 Cell Biolabs 96 assays
EUR 856
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit

STA-416-5 Cell Biolabs 5 x 96 assays
EUR 3524
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)

ACT-SL-96 ACTGene
EUR 647

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED

P-DW-12-HC-S CORNING 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96-well Plasmid DNA Extraction Kit (1 plate)

FAPWE-96001 Favorgen 1 prep
EUR 146

96 Well Plate RNA Cleanup and Concentration Kit

BRC1251 Bio Basic 2xPlates, 192prep
EUR 250.31

96-Well Plate Animal Genomic DNA Miniprep Kit

BS437 Bio Basic 5XPlates, 480prep
EUR 362.98

96-Well Plate Animal Genomic DNA Miniprep Kit

BS4372 Bio Basic 2XPlates, 192prep
EUR 195.5

96 Well Plate Yeast Genomic DNA Miniprep Kit

BS8357 Bio Basic 2XPlates, 192prep
EUR 195.5

96-Well Plate Plant Genomic DNA Miniprep Kit

BS8361 Bio Basic 2XPlates, 192prep
EUR 195.5

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 Bio Basic 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 Bio Basic 5Plates, 480prep
EUR 324.92

Block for 96 well plate, WSC-2620/30

4002659 Atto 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1292 Bio Basic 2XPlates, 192prep
EUR 175.71

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1295 Bio Basic 5XPlates, 480prep
EUR 324.92

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)

CT366 U-CyTech 5
EUR 224

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)

EXOEL-96P-1 SBI 10 Plates
EUR 213

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)

CBA-081 Cell Biolabs 96 assays
EUR 606
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-well Checkpoint Kinase Activity Assay Kit

STA-414 Cell Biolabs 96 assays
EUR 757
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit

STA-414-5 Cell Biolabs 5 x 96 assays
EUR 3060
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit

25-224 Genesee Scientific 100 plates
EUR 299
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well

1018-96 Intact Genomics 1/EA
EUR 518

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML

PCR-96-AB-C-PBC CORNING 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTA55-5P Alpha Diagnostics 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTG65-5P Alpha Diagnostics 1 PK
EUR 263

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA

C075-1EA Arbor Assays 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA

C140-1EA Arbor Assays 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA

C223-1EA Arbor Assays 1EA
EUR 178

Goat anti-Mouse, Coated White 96 Well Plate, 1EA

X010-1EA Arbor Assays 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA

X014-1EA Arbor Assays 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA

X016-1EA Arbor Assays 1EA
EUR 109

2 x Black Half Area 96 Well Plate, 2EA

X037-2EA Arbor Assays 2EA
EUR 94

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA

X061-1EA Arbor Assays 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA

X063-1EA Arbor Assays 1EA
EUR 109

Adaptor for 96-well PCR plate, accessory for AgileSealer?

APS-200-96PCR ACTGene
EUR 351

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 Bio Basic 5XPlates, 480prep
EUR 487.82

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 Bio Basic 2XPlates, 192prep
EUR 250.31

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92028 Bio Basic 2XPlates, 192prep
EUR 212.25

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92029 Bio Basic 5XPlates, 480prep
EUR 364.5

Block for 96 deep-well plate, WSC-2620/30

4002661 Atto 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE

432071 CORNING 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480130 CORNING 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES

480131 CORNING 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480132 CORNING 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

96 Well PCR Plates, Fits ABI Fast Plate Machines

GP615 GenDepot 25 plates
EUR 212

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)

CBA-320 Cell Biolabs 96 assays
EUR 537
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

Clear 384 Well Plate

X105-1EA Arbor Assays 1EA
EUR 112

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL Cell Biolabs 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN Cell Biolabs 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)

CBA-220 Cell Biolabs 96 assays
EUR 635
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231 Cell Biolabs 120 assays
EUR 635
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231-5 Cell Biolabs 5 x 120 assays
EUR 2648
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Feline SAA Detection ELISA Assay Kit

6028 Chondrex 1 kit
EUR 553.15
Description: Feline SAA Detection ELISA Assay Kit

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTAG75-5P Alpha Diagnostics 1 PK
EUR 286

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA

X011-1EA Arbor Assays 1EA
EUR 102

FALCON® 96 WELL CLEAR ROUND BOTTOM NOT TREATED ASSAY PLATE, NONSTERILE, 5/PACK, 50/CASE

353910 CORNING 5/pk
EUR 91
Description: Assay - DL; Assay 96 well - DL

Mouse anti-Cystatin C Clear Coated 96 Well Plate, 1EA

C033-1EA Arbor Assays 1EA
EUR 178

Mouse anti-Endothelin-1 Clear Coated 96 Well Plate, 1EA

C161-1EA Arbor Assays 1EA
EUR 178

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)

B814152-0002 Bio Basic 2XPlates, 2XPlates
EUR 155.16

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)

B814152-0005 Bio Basic 5XPlates, 5XPlates
EUR 250.31

96 Well 0.2ml PCR Plate, Chimney-Top 10/Bag, Sterile

PCR-960-P-CH-S Bio Basic 1PK, 10UNIT
EUR 92.79

DNA-BIND™ PLATE, 96 WELL, CLEAR, FLAT 1/10

2525 CORNING 1/pk
EUR 137
Description: Genomics; Genomic Plates

Sealing film, PCR 96 Well Plate Sealing Membrane, 100/pk

P1001-PCR MTC Bio 100/pack
EUR 95.9
Description: Sealing film PCR 96 Well Plate Sealing Membrane

96 WELL CLEAR PCR MICROPLATE

PCR-96-C CORNING 10/pk
EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Black 384 Well Plate, 2EA

X115-2EA Arbor Assays 2EA
EUR 207

384 Well Plate, 10/Bag

PCR-384-SN Bio Basic 1PK, 10UNIT
EUR 93.5

Adapter for 24 well plate

3510146 Atto 1unit
EUR 5581

Tissue Culture Plate, 12 Well

TCP20-12 Bio Basic 1 UNIT
EUR 53.48

Tissue Culture Plate, 48 Well

TCP20-48 Bio Basic 1 UNIT
EUR 53.48

Tissue Culture Plate, 6 Well

TCP20-6 Bio Basic 1 UNIT
EUR 53.48

Random Nanofibers 24 Well Plate

3D00006-24 Neuromics 700 nm-PCLs
EUR 101

Random Nanofibers 348 Well Plate

3D00006-384 Neuromics 700 nm-PCLs
EUR 104

Random Nanofibers 6 Well Plate

3D00006-6 Neuromics 700 nm-PCLs
EUR 93

Aligned Nanofibers 24 Well Plate

3D00012-24 Neuromics 700 nm-PCLs
EUR 104

Aligned Nanofibers 348 Well Plate

3D00012-384 Neuromics 700 nm-PCLs
EUR 123

Aligned Nanofibers 6 Well Plate

3D00012-6 Neuromics 700 nm-PCLs
EUR 97

EZCellTM Cell Invasion Assay (laminin), 96-well, 8 µm

K914-100 Biovision
EUR 566

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric

CBA-104 Cell Biolabs 96 assays
EUR 635
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric

CBA-104-5 Cell Biolabs 5 x 96 assays
EUR 2613
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric

CBA-105 Cell Biolabs 96 assays
EUR 635
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric

CBA-105-5 Cell Biolabs 5 x 96 assays
EUR 2613
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106 Cell Biolabs 96 assays
EUR 635
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106-5 Cell Biolabs 5 x 96 assays
EUR 2613
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-Well Phagocytosis Assay (E. coli, Colorimetric Format)

CBA-222 Cell Biolabs 96 assays
EUR 693
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, E. coli Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-Well Phagocytosis Assay(Zymosan Substrate), Trial Size

CBA-224-T Cell Biolabs 20 assays
EUR 386
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE

PCR-96-FS-C-PBC CORNING 10/pk
EUR 168
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Goat anti-Mouse, 1 x 8 Strip Well, Clear Coated 96 Well Plate, 1EA

X012-1EA Arbor Assays 1EA
EUR 109

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 1x96 well plate - 20µl/well

1038-96 Intact Genomics 1/EA
EUR 518

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (5 x 96 assays)

CBA-320-5 Cell Biolabs 5 x 96 assays
EUR 2114
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

96-Well CometSlides

4253-096-03 Biotechne 1 Tests
EUR 142.4

PCR 96-Plate unskirted

A28239 GeneOn 25 pcs
EUR 98

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte

D8234148-1 Biochain 1 plate
EUR 256

96-Well 2ml Deep Plate, Natural, Edge Filled, 10 Plates/Bag

BR581-96N Bio Basic 1PK, 10UNIT
EUR 94.85

6 WELL BLUE PCR FULL SKIRT AMPLIFICATION PLATE.

PCR-96-FS-B CORNING 10/pk
EUR 176
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

CORNING® BIOCOAT™ MATRIGEL® MATRIX THIN-LAYER CLEAR FLAT BOTTOM 96-WELL ASSAY PLATE, 5/CASE

354607 CORNING 5/pk
EUR 287
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE

354730 CORNING 5/pk
EUR 85
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

96-well Plasmid ezFilter Mini Kit

K1325-96-1 Biovision
EUR 370

96-well Plasmid ezFilter Mini Kit

K1325-96-4 Biovision
EUR 968
Clarifying the precise perform of DEAD-box helicases in most cancers might be intricate, and depends upon the mobile milieu and interacting components. This overview goals to summarize the present information on the quite a few capacities which have been ascribed to DEAD-box RNA helicases. It additionally highlights their numerous actions upon malignant transformation within the numerous tumor sorts.

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Liquid Handling Equipment & Lab Consumables manufacturing company. Pipettes | Centrifuge | Tubes | Tips | Incubators | PCR Consumables We have got it all!

Accumax - Neuation
AccumaxNeuationAccumax - Neuation@AccumaxNeuation·
25 May

The versatile lab #centrifuge iFuge L400P by Neuation has more than 15 rotor options & can further be customized as per user applications. Adept to handle various applications in hospitals, clinical labs, universities, & research & industrial laboratories
https://lnkd.in/dt_AYaCP

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AccumaxNeuationAccumax - Neuation@AccumaxNeuation·
9 May

Single block dry bath iTherm D150-1 is adept to provide uniform heating to your sample with utmost accuracy.

Know more: https://lnkd.in/gVHR3ww8

#drybath #biotechnology #molecularbiology #biotech #labequipment

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AccumaxNeuationAccumax - Neuation@AccumaxNeuation·
4 May

Accumax pipettes are ergonomically designed and lightweight to give you an easy, accurate, and comfortable pipetting experience
Know more: https://lnkd.in/dea92Wr6
#laboratory #labequipment #laboratorytesting #LifeSciences

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AccumaxNeuationAccumax - Neuation@AccumaxNeuation·
23 Apr

Today we celebrate #WorldLaboratoryDay to honor the unique workspaces where great inventions are made to make the world a better place.
#laboratory #laboratoryday #accumax #neuation

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AccumaxNeuationAccumax - Neuation@AccumaxNeuation·
17 Apr

Wishing everyone a Happy Easter !!
#Easter2022 #easter

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