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Collaborative Computational Project No. 14

Collaborative Computational Project No. 14

Camel Mab stabilisation of Proteins for X-rtay christal structure detection

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Targeting the Coronavirus Nucleocapsid Protein through GSK-3 Inhibition

Posted by Darren Marshall on March 4, 2021 in Antibodies, Assay Kits, Biology Cells, Blog, cDNA, Clia Kits, Culture Cells, Devices, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, RNA, Simultaneous Enhancement of Near-Infrared Emission and Dye Photodegradation in a Racemic Aspartic Acid Compound via Metal-Ion Modification., Synthesis of Highly Functionalized 2-Pyranone from Silyl Ketene., Thermodynamic and kinetic studies of H2 and N2 binding to bimetallic nickel-group 13 complexes and neutron structure of a Ni(ฮท2-H2) adduct., Western Blot
Targeting the Coronavirus Nucleocapsid Protein through GSK-3 Inhibition

The coronaviruses chargeable for extreme acute respiratory syndrome (SARS-CoV), COVID-19 (SARS-CoV-2), Middle East respiratory syndrome (MERS-CoV), and different coronavirus infections specific a nucleocapsid protein (N) that’s important for viral replication, transcription, and virion meeting. Phosphorylation of N from SARS-CoV by glycogen synthase kinase 3 (GSK-3) is required for its operate and inhibition of GSK-3 with lithium impairs N phosphorylation, viral transcription, and replication. Here we report that the SARS-CoV-2 N protein accommodates GSK-3 consensus sequences and that this motif is conserved in numerous coronaviruses, regardless of restricted total sequence conservation, elevating the risk that SARS- CoV-2 could also be delicate to GSK-3 inhibitors together with lithium.

We performed a retrospective evaluation of lithium use in sufferers from three main well being techniques who had been PCR examined for SARS-CoV-2. We discovered that sufferers taking lithium have a considerably decreased danger of COVID- 19 (odds ratio = 0.51 [0.34 – 0.76], p = 0.001). We additionally present that the SARS-CoV-2 N protein is phosphorylated by GSK-3. Knockout of GSK3A and GSK3B demonstrates that GSK-3 is crucial for N phosphorylation. Alternative GSK-3 inhibitors block N phosphorylation and impair replication in SARS-CoV-2 contaminated lung epithelial cells in a cell-type dependent method. Targeting GSK-3 could due to this fact present a brand new method to deal with COVID-19 and future coronavirus outbreaks.

Increasing numbers of protein interactions have been recognized in high-throughput experiments, however solely a small proportion have solved constructions. Recently, sequence coevolution-based approaches have led to a breakthrough in predicting monomer protein constructions and protein interplay interfaces. Here, we tackle the challenges of large-scale interplay prediction at residue decision with a quick alignment concatenation technique and a probabilistic rating for the interplay of residues. Importantly, this technique (EVcomplex2) is ready to assess the chance of a protein interplay, as we present right here utilized to large-scale experimental datasets the place the pairwise interactions are unknown. We predict 504 interactions de novo in the E. coli membrane proteome, together with 243 which might be newly found. While EVcomplex2 doesn’t require obtainable constructions, coevolving residue pairs can be utilized to supply structural fashions of protein interactions, as performed right here for membrane complexes together with the Flagellar Hook-Filament Junction and the Tol/Pal advanced.

The analysis and administration of allergic reactions in sufferers sensitized to non-specific Lipid Transfer Proteins

Sensitization to a number of non-specific lipid switch proteins(nsLTP), initially thought to exist primarily in southern Europe,is changing into accepted as a reason behind allergic reactions to plant meals throughout Europe and past.The peach nsLTP allergen Pru p Three is a dominant sensitizing allergen, and peaches a standard meals set off, though a number of meals may be concerned. A frequent characteristic of reactions is the requirement for a co-factor (train, alcohol, non-steroidal anti-inflammatory medication, Cannabis sativa)to be current for a meals to elicit a response. The variability in the meals and co-factor triggers make it important to incorporate an allergy targeted food regimen and scientific historical past in the diagnostic work-up.

Testing on suspected meals triggers, also needs to set up whether or not sensitization to nsLTP is current, usingpurified or recombinant nsLTP allergens comparable to Pru p 3. Theavoidance of recognized set off meals and recommendation on co-factors is presently the primary administration for this situation. Studies on immunotherapy are promising, however it’s unknown whether or not such therapies will probably be helpful in populations the place Pru p Three is just not the major sensitizing allergen. Future analysis ought to give attention to the mechanisms of co-factors, bettering diagnostic accuracy and establishing the efficacy of immunotherapy.Targeting the Coronavirus Nucleocapsid Protein through GSK-3 Inhibition

Evaluation of Fused Pyrrolothiazole Systems as Correctors of Mutant CFTR Protein

Cystic fibrosis (CF) is a genetic illness attributable to mutations that impair the operate of the CFTR chloride channel. The most frequent mutation, F508del, causes misfolding and untimely degradation of CFTR protein. This defect may be overcome with pharmacological brokers named “correctors”. So far, at the very least three completely different courses of correctors have been recognized based mostly on the additive/synergistic results which might be obtained when compounds of various courses are mixed collectively. The improvement of sophistication 2 correctors has lagged behind that of compounds belonging to the different courses.

It was proven that the efficacy of the prototypical class 2 corrector, the bithiazole corr-4a, could possibly be improved by producing conformationally-locked bithiazoles. In the current examine, we investigated the impact of tricyclic pyrrolothiazoles as analogues of constrained bithiazoles. Thirty-five compounds had been examined utilizing the purposeful assay based mostly on the halide-sensitive yellow fluorescent protein (HS-YFP) that measured CFTR exercise. One compound, having a six atom carbocyle central ring in the tricyclic pyrrolothiazole system and bearing a pivalamide group at the thiazole moiety and a 5-chloro-2-methoxyphenyl carboxamide at the pyrrole ring, considerably elevated F508del-CFTR exercise. This compound might result in the synthesis of a novel class of CFTR correctors.

Mouse antibody for Feline Leukemia virus FeLv gp70

7217 Virostat 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv gp70 for WB, ELISA.

Mouse antibody for Feline Leukemia virus FeLv p27

7218 Virostat 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Mouse antibody for Feline Leukemia virus FeLv p27

7226 Virostat 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Mouse antibody for Feline Leukemia virus FeLv p27

7228 Virostat 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Feline Leukemia virus FeLv p27 for WB, ELISA.

Feline Leukemia virus antibody

10C-CR1297M1 Fitzgerald 100 ug
EUR 247
Description: Mouse monoclonal Feline Leukemia virus antibody

Feline FeLV antibody

10-2328 Fitzgerald 100 ug
EUR 325
Description: Mouse monoclonal Feline FeLV antibody for lateral flow

Feline FeLV antibody

10-2329 Fitzgerald 100 ug
EUR 325
Description: Mouse monoclonal Feline FeLV antibody for lateral flow

Feline Leukaemia Virus gp70 (FeLV gp70) Antibody

abx413483-025mg Abbexa 0.25 mg
EUR 592

Feline Leukaemia Virus p15E (FeLV p15E) Antibody

abx414340-025mg Abbexa 0.25 mg
EUR 676

Feline Leukaemia Virus p27 (FeLV p27) Antibody

abx414341-025mg Abbexa 0.25 mg
EUR 676

Feline Leukemia Virus p27 antibody

10-1520 Fitzgerald 100 ug
EUR 337
Description: Mouse monoclonal Feline Leukemia Virus p27 antibody

Feline Leukemia virus p27 antibody

10-F05A Fitzgerald 250 ug
EUR 308
Description: Mouse monoclonal Feline Leukemia virus antibody

Feline Leukemia Virus p27 antibody

10-F05C Fitzgerald 250 ug
EUR 217
Description: Mouse monoclonal Feline Leukemia Virus p27 antibody

Feline Leukemia Virus gp70 antibody

10-F07A Fitzgerald 250 ug
EUR 327
Description: Mouse monoclonal Feline Leukemia Virus gp70 antibody

Feline Leukemia Virus gp70 antibody

10-F07B Fitzgerald 250 ug
EUR 327
Description: Mouse monoclonal Feline Leukemia Virus gp70 antibody

Feline Leukemia Virus p27 antibody

20-FG32 Fitzgerald 1 mg
EUR 128
Description: Goat polyclonal Feline Leukemia Virus p27 antibody

Feline leukemia virus PCR kit

PCR-V176-48R Bioingentech 50T
EUR 823

Feline leukemia virus PCR kit

PCR-V176-96R Bioingentech 100T
EUR 1113

Feline Leukemia Virus p27 protein

30-1928 Fitzgerald 100 ug
EUR 457
Description: Recombinant FeLV p27 protein with his tag

Feline Leukemia Virus p27 antibody (HRP)

60-5006 Fitzgerald 1 ml
EUR 409
Description: Goat polyclonal Feline Leukemia Virus p27 antibody (HRP)

Feline Leukemia Virus p27 antibody (biotin)

60-5007 Fitzgerald 1 ml
EUR 378
Description: Goat polyclonal Feline Leukemia Virus p27 antibody (biotin)

Feline leukemia virus RT PCR kit

RTq-V176-100R Bioingentech 100T
EUR 1311

Feline leukemia virus RT PCR kit

RTq-V176-150R Bioingentech 150T
EUR 1787

Feline leukemia virus RT PCR kit

RTq-V176-50R Bioingentech 50T
EUR 963

Goat polyclonal antibody for Feline Leukemia virus

274 Virostat 1 ml
EUR 321.88
Description: This is HRP conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus

7221 Virostat 1 ml
EUR 283.81
Description: This is goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus

7223 Virostat 1 ml
EUR 300.13
Description: This is FITC conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Goat polyclonal antibody for Feline Leukemia virus

7227 Virostat 1 ml
EUR 300.13
Description: This is Biotin conjugated goat polyclonal antibody against Feline Leukemia virus p27 for WB, ELISA.

Feline leukemia virus One-Step PCR kit

Oneq-V176-100R Bioingentech 100T
EUR 1610

Feline leukemia virus One-Step PCR kit

Oneq-V176-150R Bioingentech 150T
EUR 2205

Feline leukemia virus One-Step PCR kit

Oneq-V176-50R Bioingentech 50T
EUR 1175

Bovine Leukemia Virus(Leukemia Virus)

QY-E60121 Qayee Biotechnology 96T
EUR 426

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody

20-abx322439 Abbexa
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody

20-abx322440 Abbexa
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVC2) Antibody

abx034469-400ul Abbexa 400 ul
EUR 523

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVC2) Antibody

abx034469-80l Abbexa 80 ยตl
EUR 286

Feline Leukemia Virus Subgroup C Receptor-Related Protein 2 (FLVCR2) Antibody

20-abx006544 Abbexa
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Feline Panleukopenia Virus PCR kit

PCR-V171-48D Bioingentech 50T
EUR 453

Feline Panleukopenia Virus PCR kit

PCR-V171-96D Bioingentech 100T
EUR 572

Feline immunodeficiency virus PCR kit

PCR-V175-48R Bioingentech 50T
EUR 823

Feline immunodeficiency virus PCR kit

PCR-V175-96R Bioingentech 100T
EUR 1113

Feline Herpes Virus Type 1 (FHV1) Antibody

abx414268-025mg Abbexa 0.25 mg
EUR 676

FeLV

AG20-0281-Z Immunology Consultants Laboratory 0.1 mg
EUR 349

Feline Panleukopenia Virus RT PCR kit

RTq-V171-100D Bioingentech 100T
EUR 717

Feline Panleukopenia Virus RT PCR kit

RTq-V171-150D Bioingentech 150T
EUR 808

Feline Panleukopenia Virus RT PCR kit

RTq-V171-50D Bioingentech 50T
EUR 598

Feline immunodeficiency virus RT PCR kit

RTq-V175-100R Bioingentech 100T
EUR 1311

Feline immunodeficiency virus RT PCR kit

RTq-V175-150R Bioingentech 150T
EUR 1787

Feline immunodeficiency virus RT PCR kit

RTq-V175-50R Bioingentech 50T
EUR 963

Recombinant Feline Immunodeficiency Virus Core Antigen

DAG552 Creative Diagnostics 100ug
EUR 949

Native Feline Infectious Peritonitis Virus antigen

DAGA-3038 Creative Diagnostics 1mg
EUR 1040

Feline Immunodef. virus core recombinant antigen

8963 Virostat 100 ug
EUR 561.13
Description: This is Feline Immunodef. virus core recombinant antigen for ELISA,WB.

Feline Immunodeficiency virus core Recombinant Protein

53-312 ProSci 0.01 mg
EUR 710.75
Description: Feline Immunodeficiency virus core Recombinant Protein

Mouse Leukemia virus protein

30-1273 Fitzgerald 1 mg
EUR 241
Description: Purified native Mouse Leukemia virus protein

FeLV gp70 Antibody

abx021714-1mg Abbexa 1 mg
EUR 1295

FeLV p30 Antibody

abx022983-1ml Abbexa 1 ml
EUR 578

Anti-FeLV antibody

10-2801 Fitzgerald 1 mg
EUR 181
Description: Anti-Feline Leukemia Virusย antibody

Anti-FeLV antibody

10-2802 Fitzgerald 1 mg
EUR 181
Description: Anti-Feline Leukemia Virus antibody

Anti-FeLV antibody

10-2803 Fitzgerald 1 mg
EUR 181
Description: Anti-Feline Leukemia Virus antibody

Mouse Anti Feline Leukaemia Virus P15e Monoclonal Antibody

DMABT-51278MF Creative Diagnostics 0.25 mg
EUR 767

Mouse Anti Feline Leukaemia Virus P27 Monoclonal Antibody

DMABT-51280MF Creative Diagnostics 0.2 mg
EUR 767

Mouse Anti Feline Leukaemia Virus P27 Monoclonal Antibody

DMABT-51281MF Creative Diagnostics 0.25 mg
EUR 767

Goat Anti Feline Leukaemia Virus P27 Polyclonal Antibody

DPBT-66847GF Creative Diagnostics 1 ml
EUR 861

Feline Immunodeficiency Virus p24 Antigen (FIV p24) Antibody

abx411336-02mg Abbexa 0.2 mg
EUR 592

Feline Immunodeficiency Virus gp41 Antigen (FIV gp41) Antibody

abx411337-02mg Abbexa 0.2 mg
EUR 592

Feline Immunodeficiency Virus p24 Antigen (FIV p24) Antibody

abx412988-2ml Abbexa 2 ml
EUR 606

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody

20-abx312919 Abbexa
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody

20-abx323336 Abbexa
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody

20-abx104171 Abbexa
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody

20-abx104172 Abbexa
  • EUR 300.00
  • EUR 133.00
  • EUR 801.00
  • EUR 425.00
  • EUR 258.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody

abx011224-100ug Abbexa 100 ug
EUR 411

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody

20-abx327526 Abbexa
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Feline Panleukopenia Virus One-Step PCR kit

Oneq-V171-100D Bioingentech 100T
EUR 866

Feline Panleukopenia Virus One-Step PCR kit

Oneq-V171-150D Bioingentech 150T
EUR 981

Feline Panleukopenia Virus One-Step PCR kit

Oneq-V171-50D Bioingentech 50T
EUR 718

Feline immunodeficiency virus One-Step PCR kit

Oneq-V175-100R Bioingentech 100T
EUR 1610

Feline immunodeficiency virus One-Step PCR kit

Oneq-V175-150R Bioingentech 150T
EUR 2205

Feline immunodeficiency virus One-Step PCR kit

Oneq-V175-50R Bioingentech 50T
EUR 1175

Feline Immunodeficiency Virus (F1a), RNA Aptamer, unlabeled

AR-230-U Alpha Diagnostics Custom Ask for price

Feline Immunodeficiency Virus gp95 Envelope Antigen (FIV gp95) Antibody

abx411335-02mg Abbexa 0.2 mg
EUR 592

Feline Immunodeficiency Virus gp95 Envelope Antigen (FIV gp95) Antibody

abx414342-025mg Abbexa 0.25 mg
EUR 592

Bovine leukemia virus PCR kit

PCR-V221-48R Bioingentech 50T
EUR 823

Bovine leukemia virus PCR kit

PCR-V221-96R Bioingentech 100T
EUR 1113

Cat Leukemia Virus p27 Protein

20-abx260224 Abbexa
  • EUR 885.00
  • EUR 342.00
  • EUR 1609.00
  • 0.5 mg
  • 100 ug
  • 1 mg

Friend Leukemia Virus Integration 1 (FLI1) Antibody

20-abx131009 Abbexa
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Friend Leukemia Virus Integration 1 (FLI1) Antibody

20-abx112594 Abbexa
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Moloney murine leukemia virus Gag polyprotein Antibody

20-abx110606 Abbexa
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody (HRP)

20-abx312920 Abbexa
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody (FITC)

20-abx312921 Abbexa
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Myeloproliferative Leukemia Virus Oncogene (MPL) Antibody (Biotin)

20-abx312922 Abbexa
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Friend Leukemia Virus Integration 1 (FLI1) Antibody

20-abx338832 Abbexa
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Friend Leukemia Virus Integration 1 (FLI1) Antibody

20-abx004315 Abbexa
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Friend Leukemia Virus Integration 1 (FLI1) Antibody

abx145181-100ug Abbexa 100 ug
EUR 391

Friend Leukemia Virus Integration 1 (FLI1) Antibody

20-abx009258 Abbexa
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Friend Leukemia Virus Integration 1 (FLI1) Antibody

20-abx013081 Abbexa
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 ยตg

Friend Leukemia Virus Integration 1 (FLI1) Antibody

abx032839-400ul Abbexa 400 ul
EUR 523

Friend Leukemia Virus Integration 1 (FLI1) Antibody

abx032839-80l Abbexa 80 ยตl
EUR 286

Friend Leukemia Virus Integration 1 (FLI1) Antibody

abx233158-100ug Abbexa 100 ug
EUR 509
×

Currently, there’s a debate concerning whether or not supply of protein differentially impacts musculoskeletal well being in older adults. Whey and soy protein have been used as the most typical high-quality proteins in current literature. However, there may be rising shopper demand for extra plant-sourced dietary protein choices. For instance, pea protein is quickly gaining reputation amongst shoppers, regardless of little to no analysis concerning its long-term affect on muscle well being. Therefore, the goals of this evaluate are to: (1) evaluate present literature from the previous decade evaluating whether or not particular supply(s) of dietary protein present most profit to muscle well being in older adults; and (2) spotlight the want for future analysis particular to underrepresented plant protein sources, comparable to pea protein, to then present clearer messaging surrounding plant-sourced versus animal-sourced protein and their results on the ageing musculoskeletal system.

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The first day at Analytica, Germany - Meet our team @ Stand no B1.108
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