Immunofluorescence Workflow

Tissue preparation

 VECTABOND adhesive for tissue sections
 ImmPrint histological pen
 ImmEdge hydrophobic barrier pen

Antigen unmasking

 Antigen unmasking solutions, based on citrate or Tris

Extinguishing / blocking

 TrueVIEW autofluorescence kit **
 TrueVIEW kit for autofluorescence quenching with DAPI **
 Avidin / biotin blocking kit
 Streptavidin / biotin blocking kit
 Normal serum
 Animal-Free Blocker® and thinner
⯈ Casein solution
 MOM (mouse to mouse) blocking agent
 Carbo-Free ™ blockers
 BLOXALL solution for blocking endogenous HRP / AP

Primary antibodies / lectins *

 Biotinylated lectins
 Fluorochrome conjugated lectins
 Unconjugated lectins



 VectaFluor DyLight RTU secondary antibody
 VectaFluor Duet IF kits for double marking
 MOM (Mouse to Mouse) immunodetection kits
 Biotinylated secondary antibodies
 Fluorochrome-conjugated secondary antibodies
 Unconjugated secondary antibodies

Tertiary reagent

 VectaFluor Excel DyLight amplified fluorescent staining systems
 Fluorochrome conjugated to avidin or streptavidin
 Biotinylated anti-avidin amplification reagent
 Biotinylated anti-streptavidin amplification reagent

Contrast staining /


⯈ VECTASHIELD Antifade mounting medium
⯈ VECTASHIELD Antifade mounting medium with DAPI
⯈ VECTASHIELD Antifade mounting medium with propidium iodide (PI)
 VECTASHIELD HardSet Antifade mounting medium
 VECTASHIELD HardSet Antifade mounting medium with DAPI
 VECTASHIELD HardSet Antifade mounting medium with TRITC-phaloidin
ECT VECTASHIELD Vibrance Antifade mounting medium
 VECTASHIELD Vibrance Antifade mounting medium with DAPI



The new regulatory RNAs: circRNAs

The RNA circular (circRNAs) are a type of non – coding RNA newly discovered and opens a promising new field of research.
They are RNA molecules with a closed ring shape that maintains their structure thanks to covalent bonds. The vast majority of these circRNAs originate from exons and are highly conserved across species.
Among its main characteristics are its high stability, much higher than that of linear RNA due to resistance to nuclease degradation, and its abundant presence in the cytoplasm. But, without a doubt, the most relevant is the high presence in its sequence of target sites for microRNAs.
Due to all these characteristics, circRNAs have been gaining scientific relevance due to their role as “miRNA sponges” , a type of so-called “competitive endogenous RNA (ceRNAs)” that would exercise their regulatory function by hijacking miRNAs and preventing them from having instead its natural mRNA inhibitory function.
The differential expression of these circRNAs in tissues and / or cells at different stages of development, together with their long half-life, proposes them as possible candidates for biomarkers in various diseases or developmental control.
Arraystar exclusively offers the analysis of the expression of circRNAs by means of microarrays, for both human and mouse samples. All you have to do is send us your samples, and the Arraystar team of experts will carry out the test, a complete and detailed bioinformatic analysis and send you the results.