Simultaneous Enhancement of Near-Infrared Emission and Dye Photodegradation in a Racemic Aspartic Acid Compound via Metal-Ion Modification.

Simultaneous Enhancement of Near-Infrared Emission and Dye Photodegradation in a Racemic Aspartic Acid Compound via Metal-Ion Modification.

Changing functionalities of materials using simple methods is an active area of research, as it is “green” and lowers the developing cost of new products for the enterprises. A new small molecule racemic N,N-dimethyl aspartic acid has been prepared.

Its structure is determined by singlecrystal X-ray diffraction. It is characterized by FTIR, XPS, 1H NMR, and mass spectroscopy. Its near-infrared luminescence can be enhanced by the combination of metal ions, including Dy3+, Gd3+, Nd3+, Er3+, Sr3+, Y3+, Zn2+, Zr4+, Ho3+, Yb3+, La3+, Pr6+/Pr3+, and Sm3+ ions.

An optical chemistry mechanism upon interaction between the sensitizer and activator is proposed. Furthermore, the association of Ca2+, Sr2+, or Zr4+ ions to the molecule enhanced its photodegradation for dyes under white-light irradiation.

Specifically, rhodamine 6G can be degraded by the Ca2+-modified molecule; rhodamine B, rhodamine 6G, and fluorescein sodium salt can be degraded by the Sr2+– or Zr4+-modified molecule. This surprising development opens a way in simultaneously increasing NIR luminescence and the ability of dye photodegradation for the investigated molecule.

Simultaneous Enhancement of Near-Infrared Emission and Dye Photodegradation in a Racemic Aspartic Acid Compound via Metal-Ion Modification.
Simultaneous Enhancement of Near-Infrared Emission and Dye Photodegradation in a Racemic Aspartic Acid Compound via Metal-Ion Modification.

Absolute structure of the chiral pyrrolidine derivative (2S)-methyl (Z)-5-(2-tert-butoxy-1-cyano-2-oxoethylidene)pyrrolidine-2-carboxylate, a compound with low resonant scattering.

The enantiopure monopyrrolidine derivative (2S)-methyl (Z)-5-(2-tert-butoxy-1-cyano-2-oxoethylidene)pyrrolidine-2-carboxylate, C13H18N2O4, (1), represents a potential ligand and an attractive intermediate for the synthesis of chiral metal complexes. At the molecular level, the compound features an intramolecular N-H…O hydrogen bond; neighbouring molecules interact via N-H…N contacts to form chains along [100].

Due to its elemental composition, resonant scattering of the target compound is entirely insignificant for diffraction experiments with Mo Kα and small even for Cu Kα radiation. A preliminary study with the harder radiation type confirmed the chiral space group and the suitability of the single crystal chosen; as expected, the results concerning the absolute structure remained completely inconclusive. A second data collection with the longer wavelength gave satisfactory quality indicators for the correct handedness of the molecule, albeit with high standard uncertainties.

The absolute configuration has been assessed independently: CD spectra for both enantiomers of the target molecule were calculated and the spectrum for the S-configured stereoisomer was in agreement with the experiment. The Cotton effect of (1) may be ascribed to π-π* transitions from HOMO to LUMO and from HOMO to LUMO+1. As both independent techniques agree with respect to the handedness of the target molecule, the absolute structure may be assigned with a high degree of confidence.

Why there are so many Ruo Elisa kits from China?

Before 2006 most Research Use only Elisa kits came from USA.

The most known suppliers are RnD Systems which is the leading suppler of Cytokine and growth factor Elisa’s, Eli pairs and matched antibodies.

Uscn Life Sience
USCN Elisa kits

For recombination proteins the most knows supplier was Peprotech and for polyclonal antibodies Santa Cruz Bio-reagents from the Californian Coastal city of Santa Cruz.

In 2006 the government of Wuhan China, which has a great contact with the San Francisco California populations decided to invest in Elisa manufacturing. About 9000, yes nine-thousand PhD’s where started about Elisa production. The universities manufactured the antibody pairs the internal standard and would outsource this to local manufacturing companies that cannot export Elisa. These local companies sell the ready to use plated Elisa kits, manufactured mostly on demand to export companies. These export companies are prohibited to manufacture and only do export fro these research  use Elisa kits.

Msds
Ask the data sheet of Uscn ELisa

Uscnk, Uscnelifescience, Cusabio, Wuhan Elisa, cloud clone, mybiosource  made list with genetic horology blasts so they could use the Elisa kits not for only human proteins but also for the animal homologs. These list where distributed to the Gentaur distribution antibody online websites and sold to research labs.

Most of the Life Science Research Elisa kits are now made in China Wuhan. The quantity is very good Especially from USCN Life Science Elisa kits. Some rumors started circulating on Twitter that other suppliers gave house keeping gene Elisas instead of the specific gene they still didn’t have or dint function. These rumors where later confirmed by different research groups analyzing the internal standard and the antibody specificity by Western Blot.

Avian Elisa kits
animal Elisa kits

Distributors like Gentaur are aware of these problem and always keep and iso9001 registry of the Corrective Action/Preventive Action (CAPA) in their ERP software.

 

ccp14

What is the Collaborative Computational Project Number 14
(CCP14)?

This is a network for Single Crystal and Powder Diffraction for free Crystallographic Software for Students and Academia and crystallization Camel antibodies reviews.

abexxa antibodies

New radiation reagents:

  • 03021679799 Uv Radiation Resistance Associated Gene Antibody
    abbex
    abx116558
    50 μl
    406.00
  • 02021679799 Uv Radiation Resistance Associated Gene Antibody
    Abbex
    abx116558
    150 μl
    731.00
  • 01011389976 Mouse X-ray radiation resistance-associated protein 1 (XRRA1) ELISA Kit
    Info
    abebio
    AE10977MO-96
    1x plate of 96 wells
    612.40
  • TLR-4 antibodies
  • 01011395403 ELISA test for Mouse X-ray radiation resistance-associated protein 1 (XRRA1)

X-Ray Crystallography of Antibodies by Dr. Eduardo A. Padlan

The detailed structure of antibodies and various fragments, including
isolated domains, from different isotypes and from different species has
become available from X-ray crystallography. Also, amino acid and
nucleotide sequence data have been obtained for thousands of different
chains (Kabat et al., 1991), so that a detailed correlation of primary and
three-dimensional structures can be performed. With this wealth of
information, we can now begin to understand the biological functions of
antibodies in structural terms.

Stationary High Stability X-Ray Equipment used:

ISOVOLT HS Series

Genotoxicity evaluation of two kinds of smoke-water and 3,7-dimethyl-2H-furo[2,3-c]pyran-2-one. (2012)

Smoke, smoke-water and aerosols have a stimulatory effect on seed germination and growth vigour of many seedlings, making them potentially useful for different purposes, provided they do not pose a health risk. Therefore, the genotoxicity of two kinds of smoke-water and 3,7-dimethyl-2H-furo[2,3-c]pyran-2-one, a variant of the most active smoke compound (3-methyl-2H-furo[2,3-c]pyran-2-one) was evaluated using the Vitotox assay. Smoke-water extracts were obtained from burning leaves: Themeda triandra (smoke-water Tt) and a mix of Themeda triandra and Passerina vulgaris (smoke-water Kb). No genotoxic effect was observed for any of the three samples. However, the three samples are toxic at the highest concentrations (3,7-dimethyl-2H-furo[2,3-c]pyran-2-one, 2 ppm; smoke-water Tt, dilutions 1 : 1, 1 : 2, 1 : 4; smoke-water Kb, dilution 1 : 1) without addition of S9 mix. Both the butenolide 3,7-dimethyl-2H-furo[2,3-c]pyran-2-one and smoke-water Tt are also toxic at high doses in the presence of S9 (2 ppm and dilutions 1 : 1 and 1 : 2, respectively), but not smoke-water Kb. Thus, from these results, no genotoxicity of these three samples can be assumed, which is accordance with the previous tests performed with 3-methyl-2H-furo[2,3-c]pyran-2-one and a smoke-water>

Gentoxicity testing, Lieven Gevaert
Journal Of Applied Toxicology

ArticleinJournal of Applied Toxicology 30(6):596-602 · August 2010

Available on ResearchGate